Molecular Biology And Biochemistry 4C at University Of York | Flashcards & Summaries

Lernmaterialien für Molecular Biology and Biochemistry 4C an der University of York

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Which is the name for genes which are tightly packed and not accessible

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heterochromatin

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Examples of enzymes in industrial and domestic processes 

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- lipases and proteases present in washing powder to break down fats and proteins

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What is the bond between molecules in nucleosides?

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N-glycosidic

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Are nucleotides bonded by a hydrolysis or condensation reaction?

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Condensation 

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Which direction are nucleotides joined together?

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The 5' to 3' direction

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Which two molecules makes DNA more unstable in aqueous solutions?

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Oxygen and Nitrogen, this is because they will likely form hydrogen bonds which will be likely broken in the presence of water

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Why does DNA twist?

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Due to the phosphate repulsion between pi bonds 

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Where are pi bonds found and how do they increase stability?

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Pi bonds are found between stacked bases and the sharing of electrons increases stability

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Will proteins interact with the major or minor groove?

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They will interact with the major groove because there are 3 identification points

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Increasing the salt concentration in a solution containing DNA, does what?

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It will neutralise the negative charge on the phosphate and increase base stacking. 

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When DNA reanneals which factors affect the temperature required to reanneal?

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- More GC bonds 

- Longer pieces of DNA

- Ionic strength

- pH

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What can nucleosides be used for?

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Anticancer and antiviral drugs (they incorporate into our DNA and prohibit replication)

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Q:

Which is the name for genes which are tightly packed and not accessible

A:

heterochromatin

Q:

Examples of enzymes in industrial and domestic processes 

A:

- lipases and proteases present in washing powder to break down fats and proteins

Q:

What is the bond between molecules in nucleosides?

A:

N-glycosidic

Q:

Are nucleotides bonded by a hydrolysis or condensation reaction?

A:

Condensation 

Q:

Which direction are nucleotides joined together?

A:

The 5' to 3' direction

Mehr Karteikarten anzeigen
Q:

Which two molecules makes DNA more unstable in aqueous solutions?

A:

Oxygen and Nitrogen, this is because they will likely form hydrogen bonds which will be likely broken in the presence of water

Q:

Why does DNA twist?

A:

Due to the phosphate repulsion between pi bonds 

Q:

Where are pi bonds found and how do they increase stability?

A:

Pi bonds are found between stacked bases and the sharing of electrons increases stability

Q:

Will proteins interact with the major or minor groove?

A:

They will interact with the major groove because there are 3 identification points

Q:

Increasing the salt concentration in a solution containing DNA, does what?

A:

It will neutralise the negative charge on the phosphate and increase base stacking. 

Q:

When DNA reanneals which factors affect the temperature required to reanneal?

A:

- More GC bonds 

- Longer pieces of DNA

- Ionic strength

- pH

Q:

What can nucleosides be used for?

A:

Anticancer and antiviral drugs (they incorporate into our DNA and prohibit replication)

Molecular Biology and Biochemistry 4C

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