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What is transcription?
Is the first step in expressing information
DNA --> RNA
messenger RNAs (mRNAs)
encode the amino acid sequence of one or more polypeptides specified by a gene or set of genes.
What are the three MAJOR types of RNA (from transcription)?
messenger RNAs (mRNAs)
Transfer RNAs (tRNAs)
Ribosomal RNAs (rRNAs)
Pol ll RNA products are?
mRNA, microRNAs, noncoding RNA
Pol lll RNA products are?
tRNA
small 5S and 7SL
What striking difference between the two polymerases (bacterial and eukaryotic) can you identify?
The eukaryotic enzyme has an additional c-terminal domain, which is phosphorylated.
What proteins do the phosphorylating in polymerases?
Kinases
What kind of proteins do de-phosphorylation in polymerases?
Phosphotases
How could repressors inhibit transcription?
Repressor proteins bind to the repressor binding sites, inhibiting RNA polymerase to transcribe the DNA.
Activators bind to activators-binding site, facilitating transcription by facilitating the binding of RNA polymerase to the promoter.
Which of the following must occur in order for a repressible operon to be transcribed?
RNA polymerase must bind to the promoter, and the repressor must be inactive
What are the polymerases in eukaryotes?
Pol II
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