Biochemie - Nucleinsäuren at LMU München

Flashcards and summaries for Biochemie - Nucleinsäuren at the LMU München

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Ribonucleotid-Reduktase

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Bindung zwischen den Nucleinsäuren 

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Thymidylat-Synthase

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Exemplary flashcards for Biochemie - Nucleinsäuren at the LMU München on StudySmarter:

Dihydrofolat-Reductase

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Serin-Hydroxymethyltransferase

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Base + Zucker 

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Base, Zucker + Phosphat

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Pyrimidine

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Purine

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Methylcytosin

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Zuckerverbindung in den Nucleosiden

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B-Form

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Exemplary flashcards for Biochemie - Nucleinsäuren at the LMU München on StudySmarter:

Biochemie - Nucleinsäuren

Ribonucleotid-Reduktase

  • Synthetisiert Desoxyribonucleotide aus Ribonucleotiden 
  • ersetzt die 2'-OH-Gruppe der Ribose durch ein H-Atm 
  • drei Klasse, die alle die gleiche Reaktion katalysieren
  • Reduktionsmittel ist NADPH

Biochemie - Nucleinsäuren

Bindung zwischen den Nucleinsäuren 

Phosphodiesterbindung

Biochemie - Nucleinsäuren

Thymidylat-Synthase

  • wandelt dUMP in dTMP um 
  • Methylgruppendonator ist N5-N10-Methylentetrahydrofolat
  • es entsteht Dihydrofolat, das wieder reduziert werden muss 

Biochemie - Nucleinsäuren

Dihydrofolat-Reductase

  • katalysiert die Regeneration von Tetrahydrofolat 
  • NADPH als Reduktionsmittel 
  • aus Dihydrofolat wird Tetrahydrofolat 

Biochemie - Nucleinsäuren

Serin-Hydroxymethyltransferase

  • Tetrahydrofolat wird zu Methylen-Tetrahydrofolat
  • die Methylgruppe stammt von Serin, das zu Glycin wird 
  • Cofakor: PLP

Biochemie - Nucleinsäuren

Base + Zucker 

Nukleotid 

Biochemie - Nucleinsäuren

Base, Zucker + Phosphat

Nucleotid

Biochemie - Nucleinsäuren

Pyrimidine

Thymin

Cytosin

Biochemie - Nucleinsäuren

Purine

Adenin

Guanin

Biochemie - Nucleinsäuren

Methylcytosin

  • 5. Base
  • Methylgruppe an C5
  • kann aufgrund seiner Instabilität zu Thymin desaminieren
  • entsteht durch Methylierung aus Cytosin (SAM) 

Biochemie - Nucleinsäuren

Zuckerverbindung in den Nucleosiden

  • b-glykosidische Bindung 
  • N1 bei den Pyrimidinen 
  • N9 bei den Purinen 

Biochemie - Nucleinsäuren

B-Form

  • kommt unter physiologischen Bedingungen vor 
  • rechtsgängig 
  • antiparallel 
  • Durchmesser: 2 nm
  • Abstand der Basen: 0,34 nm 
  • eine Windung: 10 Basenpaare
  • Basenpaare stehen senkrecht auf das Rückgrat
  • C-2'-Endo-Konformation

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