Biochemie Enzyme 1 at LMU München

Flashcards and summaries for Biochemie Enzyme 1 at the LMU München

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Chymotrypsin

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Holoenzym

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Gibbs freie Energie (G) 
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Gibbs Helmholtz Gleichung 

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Reaktionsgeschwindigkeit 

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Aktivierungenergie 

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Übergangszustand 
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Spezifität von Enzymen 

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Oxidoredukatasen 

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Transferasen

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Hydrolasen
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Ligasen 

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Exemplary flashcards for Biochemie Enzyme 1 at the LMU München on StudySmarter:

Biochemie Enzyme 1

Chymotrypsin
  • Extrem reaktiver Serinrest (195)
  • Katalytische Triade aus Serin, Histidin 57 und Aspartat 102
  • OH-Gruppe von Serin wird zum Alkoxid
  • Nukleophiler Angriff 
  • Amid spaltet sich ab, Acyl-Enzym entsteht (Acylierung) 
  • Carbonsäure spaltet sich ab (Deacylierung) 

Biochemie Enzyme 1

Holoenzym
Katalytisch aktives Enzym

Biochemie Enzyme 1

Gibbs freie Energie (G) 
Maß für die Energie, die für das Verrichten von Arbeit verfügbar ist

Biochemie Enzyme 1

Gibbs Helmholtz Gleichung 
DG = DH - T * DS
  • H = freie Enthalpie
  • S = Freie Entropie
  • Ist DG negativ, läuft die Reaktion spontan ab (endergonisch) 
  • Ist DG = 0 ist die Reaktion im Gleichgewicht 
  • Enzyme haben hierauf keinen Einfluss

Biochemie Enzyme 1

Reaktionsgeschwindigkeit 

  • Enzyme haben hierauf Einfluss
  • Nach einer gewissen Zeit stellt sich ein Gleichgewicht ein 
  • Wo das GG liegt, können Enzyme aber nicht beeinflussen, nur wie schnell es erreicht wird 

Biochemie Enzyme 1

Aktivierungenergie 

  • Energie, die zum Erreichen des Ubergangszustands nötig ist 
  • Wird vom Enzym gesenkt 

Biochemie Enzyme 1

Übergangszustand 

  • Zustand der höchsten Energie und Instabilität während der Reaktion
  • Wird vom Enzym stabilisiert 

Biochemie Enzyme 1

Spezifität von Enzymen 
  • Substratspezifität
  • Gruppenspezifität
  • Wirkungsspezifität
  • Optische Spezifität 

Biochemie Enzyme 1

Oxidoredukatasen 

  • Katalysieren Redoxreaktionen
  • Lactatdehydrogenase: reduziert Pyruvat zu Lactat 

Biochemie Enzyme 1

Transferasen

  • Gruppenübertragungsreaktionen
  • Z. B. ALT: transferiert die Aminogruppe von Alanin auf a-ketogkutarat, es entsteht pyruvat und Glutamat 

Biochemie Enzyme 1

Hydrolasen

  • Spaltung mittels Wasser
  • Z. B. Cymotrypsin: spaltet Peptidbindungen 
  • Z. B. Phosphatasen: Spalten p ab, Gegenteil der Kinasen

Biochemie Enzyme 1

Ligasen 

  • Verknüpfung von zwei Gruppen
  • Z. B. Aminoacyl-tRNA-Synthetase

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