Molecular Genetics at Hochschule Bonn-Rhein-Sieg

Flashcards and summaries for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg

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Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Endosome vs Lysosome vs Phagosome

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a transposon?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a Mitosis? What will come from it?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is the centromere? What is here attached?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a kinetochore? Where is it needed for what?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is satellite DNA? Properties? Where?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a micrograph?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is an oocyte?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a telomere? Where found? Properties? Use? Structure?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a genetic barcode?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a oligo dT nucleotid?
What used for? Alternative?

Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a ribozyme?

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Exemplary flashcards for Molecular Genetics at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Molecular Genetics

Endosome vs Lysosome vs Phagosome
Lysosome: containment, containing hydrolytic enzymes
Endosome: Compartment in cell with small molecules, previously internalised (prot, metabol., etc.) inside -> used for degrading
Phagosome: Compartment in cell, created by internalisation, containing a bacterium. can fuse with lysosome to form phagolysosome

Molecular Genetics

What is a transposon?
A sequence of DNA that can change its position within the DNA, sometimes with a RNA intermediate.

Molecular Genetics

What is a Mitosis? What will come from it?
Mitosis is the separation of eucaryotic cells, with 2 cells of same genetic material as result.  Not for Procaryotes! -> no nucleus

Molecular Genetics

What is the centromere? What is here attached?
A centromere is the area where the cromatides are joint in the metaphase. (no cell -division). The Kinetochores are attached here.

Molecular Genetics

What is a kinetochore? Where is it needed for what?
It is a disc shaped protein complex, attached to the centromere. Here, the spindle apparatus attaches to pull the sister chromatids apart upon cell division.

Molecular Genetics

What is satellite DNA? Properties? Where?
It names structurally simple parts of DNA with repetitions of 5-10bp of eucaryotes. It makes up up to 10% of the DNA. Most of it is situated in the heterochromatin area, close to the centromere.

Molecular Genetics

What is a micrograph?
A photo taken through a microscope

Molecular Genetics

What is an oocyte?
A female, immature egg cell

Molecular Genetics

What is a telomere? Where found? Properties? Use? Structure?
The ends of linear Chromosomes, made up of repet. For eucaryotes, six nucleotides repeat up to a thousand times. 5'–TTAGGG–3' . They stabilise the end of the chromosomes and are protein associated and have particular secondary structures.

Molecular Genetics

What is a genetic barcode?
when, for example, a library of vectors is created, one must be able to trace a gene back. To do that, a certain sequence is added to the gene of interest as a "barcode". This is done with the primer. during the PCR

Molecular Genetics

What is a oligo dT nucleotid?
What used for? Alternative?
A connected sequence of T-Nucleotides. It is used, for example for a cDNA library construction. Because one has a lot of unknown mRNAs, the primer must be unspecific. Haha! All mRNAs have a poly-A tail. This can be used as a primer attachment site. The alternative would be a random hexamer.

Molecular Genetics

What is a ribozyme?
An enzymatically active RNA fragment

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Molecular Genetics

Instrumental Analysis

IA 2 - HPLC

IA 3 - HPLC & RP-HPLC

Cell Culture

Immunology (Practical Course)

Immunology 🦠

Biochem 🧪

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Microbiology

Biochemistry

Cell Culture - Lecture 1: Basics 1

Quality Assurance EXAM PREP

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Organic Chemistry

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Immunology: B-cell Activation and Secondary Lymphoid Organs

Immunology: Antibodies and their use in research

Immunology - Chapter 4: Phagocytes

Immunology - Chapter 11: B- and T-cell development

Immunology - Chapter 8: T-cell activation in secondary lymphoid tissues

Histology

medical microbiology

Swedish

mol gen

Immunology

human biology/ histology

Immunology

Immunology

Organic Chemistry

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Cell culture - Cell lines

General Chemistry

Histology

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