Microbiology at Hochschule Bonn-Rhein-Sieg

Flashcards and summaries for Microbiology at the Hochschule Bonn-Rhein-Sieg

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Study with flashcards and summaries for the course Microbiology at the Hochschule Bonn-Rhein-Sieg

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Rhibozobium

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Streptococcus mutants (strepto = chain of cells)

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Escherichia coli
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Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

MOs can be used as producers for which recombinant proteins?

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Mycobacterium (M. Tuberculosis + leprae)

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Micrococcus luteus

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Clostridium pasteurianum + botulinum 
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Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Mycoplasma

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Fungi

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

particular DNA sequences, so-called : evolutionary chronometers:

Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Bacillus thuringiesis + subtilis
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Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

which mo is a better choice in the production of therapeutic proteins?

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Exemplary flashcards for Microbiology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Microbiology

Rhibozobium
Gram -
Habitat soil
Oxygen obligate aerobe
Shape rod
Fixes gaseous nitrogen in nodules on plant roots, doesn’t fix unless it’s in symbiotic relationship with a plant , responsible for production of Ammonia (NH4+) in nitrogencycle

Microbiology

Streptococcus mutants (strepto = chain of cells)
Gram +
Habitat mouth, part of normal flora
Oxygen -
Shape coccus
Glycoproteins of saliva form film on tooth surface, s.mutans bindsand forms microcolonies as well as biofilms in the presence of sucrose. Heavy Growth plus lactic Acid fermentation leads to demateralization of the the teeth

Microbiology

Escherichia coli
Gram -
Habitat intestine, part of normal flora
Oxygen -
Shape rod
Entirely sequenced, some belong to normal flora, but some can be pathogenic, genome easily manipulated for our benefit and typical microbe for cultivation due to fast generation time, best known enterobacteria (colonize intestine) and is distinguishable by fermentation -> endoagar

Microbiology

MOs can be used as producers for which recombinant proteins?

  • Hormones (insulin!) 
  • Vitamins 
  • Cytokines 
  • Vaccines 
  • Modified therapeutic antibodies



Microbiology

Mycobacterium (M. Tuberculosis + leprae)
Gram thick cell wall, staining with acid-fast-stain
Habitat -
Oxygen -
Shape rod
Mycolic acid gives cell wall and colonies a waxy appearance and feel, protecting it from desiccation and disinfection, extraordinary thick wall doesn’t allow it to be stained

Microbiology

Micrococcus luteus
Gram +
Habitat: Skin, dust, Soil
Oxygen:/
While diving, cells don’t properly separate, forming clusters of two or 4 cells called tetrads but no chains

Microbiology

Clostridium pasteurianum + botulinum 
Gram +
Habitat anoxic areas of soil, intestinal tract
Oxygen obligate anaerobe
Can form endospores, C.Botulinum produces toxin (neurotoxin)

Microbiology

Mycoplasma
Gram no cell wall
Habitat membranes, joints plants, soil
Oxygen -
Shape no particular
Can vary it’s shape because it has no cell wall, has thick cytoplasmic membrane instead

Microbiology

Fungi

  • eukaryotic microorganisms. 
  •  have a cell wall, are not motile, and chemoorganotroph 
  • Yeasts and Molds are subgroups of fungi

Microbiology

particular DNA sequences, so-called : evolutionary chronometers:

These are DNA-sequences which 

  • occur in all organisms which you want to compare 
  • contain conserved stretches
  • contain variable stretches 
  • Often used: 16 S/ 18 S rRNA

Microbiology

Bacillus thuringiesis + subtilis
Gram +
Habitat soil
Oxygen -
Shape rod
Can form endoscopes, use as larvicide (Bt-toxin) Gene for toxin is integrated into plant DNA to make it produce toxin itself

Microbiology

which mo is a better choice in the production of therapeutic proteins?

yeast! 

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