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Lernmaterialien für Biological Therapeutics an der Universität für Bodenkultur Wien

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TESTE DEIN WISSEN

Some people might claim, that using human embryonic stem cell lines for the production of biological therapeutics would enable the production of completely human glycosylation (incl. the ability for bi-sectioned Glc-Nac). Why is this argument not ideal?

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TESTE DEIN WISSEN

It is not ideal, because the cell line will also have lost some (god knows which) abilities for glycosylation.
Alo, the use of human cells greatly increases the risk of a contamination with pathogens that can infect humans.

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TESTE DEIN WISSEN

What is the approximate MW of a protein with 165 AA?

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TESTE DEIN WISSEN

About 19600 Da

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TESTE DEIN WISSEN

Name 2 analogs to EPO that are used in therapy!

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TESTE DEIN WISSEN
  • Eportin Alpha (Epogen)
    • EPO from CHO
  • Darbepoietin alpha (Aranesp)
    • 5AA diff from EPO -> new glycoyslation site is generated
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TESTE DEIN WISSEN

What is the most abundant cell line used in pharma USP?

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TESTE DEIN WISSEN

CHO! (70%)

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What does anapleuidie mean?

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TESTE DEIN WISSEN

It refers to an irregular number of chromosomes.

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TESTE DEIN WISSEN

The generation of recombinant cells. How is this performed differently in E.Coli vs. Mammalian cells?

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TESTE DEIN WISSEN

The process is similar, transfection or transduction can be performed.
The transfection via electroporation and plasmid will work very similar to bacterial transfection.
Still, the transduction might just potentially pose a threat for the biological therapeutic production. -> not really used. Plasmids are safer.

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TESTE DEIN WISSEN

Why can Insulin be produced in E coli?

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TESTE DEIN WISSEN

Because it only requires 3 Disulphide bonds, they can be established correctly in the DS process

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TESTE DEIN WISSEN

What are the 2 main groups of growth factors that are biotechnologically produced for human therapy?

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TESTE DEIN WISSEN

EPO stimulating agents

Colony stimulating agents

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TESTE DEIN WISSEN

Which diseases are most often treated with mABs?

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TESTE DEIN WISSEN
  • Cancers
  • Immune diseases (rheumatoid arthritis)
    • or auto immune diseases in general
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TESTE DEIN WISSEN

How many glycosylation sites are there on IgM?

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TESTE DEIN WISSEN

5

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TESTE DEIN WISSEN

Where are glycosylated Proteins produced?

Lösung anzeigen
TESTE DEIN WISSEN

E.Coli

Lösung ausblenden
TESTE DEIN WISSEN

How many O glycosylations ites are there in EPO?

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TESTE DEIN WISSEN
1
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  • 38880 Karteikarten
  • 1075 Studierende
  • 19 Lernmaterialien

Beispielhafte Karteikarten für deinen Biological Therapeutics Kurs an der Universität für Bodenkultur Wien - von Kommilitonen auf StudySmarter erstellt!

Q:

Some people might claim, that using human embryonic stem cell lines for the production of biological therapeutics would enable the production of completely human glycosylation (incl. the ability for bi-sectioned Glc-Nac). Why is this argument not ideal?

A:

It is not ideal, because the cell line will also have lost some (god knows which) abilities for glycosylation.
Alo, the use of human cells greatly increases the risk of a contamination with pathogens that can infect humans.

Q:

What is the approximate MW of a protein with 165 AA?

A:

About 19600 Da

Q:

Name 2 analogs to EPO that are used in therapy!

A:
  • Eportin Alpha (Epogen)
    • EPO from CHO
  • Darbepoietin alpha (Aranesp)
    • 5AA diff from EPO -> new glycoyslation site is generated
Q:

What is the most abundant cell line used in pharma USP?

A:

CHO! (70%)

Q:

What does anapleuidie mean?

A:

It refers to an irregular number of chromosomes.

Mehr Karteikarten anzeigen
Q:

The generation of recombinant cells. How is this performed differently in E.Coli vs. Mammalian cells?

A:

The process is similar, transfection or transduction can be performed.
The transfection via electroporation and plasmid will work very similar to bacterial transfection.
Still, the transduction might just potentially pose a threat for the biological therapeutic production. -> not really used. Plasmids are safer.

Q:

Why can Insulin be produced in E coli?

A:

Because it only requires 3 Disulphide bonds, they can be established correctly in the DS process

Q:

What are the 2 main groups of growth factors that are biotechnologically produced for human therapy?

A:

EPO stimulating agents

Colony stimulating agents

Q:

Which diseases are most often treated with mABs?

A:
  • Cancers
  • Immune diseases (rheumatoid arthritis)
    • or auto immune diseases in general
Q:

How many glycosylation sites are there on IgM?

A:

5

Q:

Where are glycosylated Proteins produced?

A:

E.Coli

Q:

How many O glycosylations ites are there in EPO?

A:
1
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