Biochemistry an der Universität Für Bodenkultur Wien | Karteikarten & Zusammenfassungen

Lernmaterialien für Biochemistry an der Universität für Bodenkultur Wien

Greife auf kostenlose Karteikarten, Zusammenfassungen, Übungsaufgaben und Altklausuren für deinen Biochemistry Kurs an der Universität für Bodenkultur Wien zu.

TESTE DEIN WISSEN

Name a few prosthetic groups


Lösung anzeigen
TESTE DEIN WISSEN
  • Flavins
  • Heme groups
  • Chlorophylls
Lösung ausblenden
TESTE DEIN WISSEN

What is the name of our enzyme version?

Lösung anzeigen
TESTE DEIN WISSEN

D152S

Lösung ausblenden
TESTE DEIN WISSEN

What kinds of methods are used to investigate the enzyme?

Lösung anzeigen
TESTE DEIN WISSEN
  • Electrophoretic methods (divide by charges -> different charges on protein and different sizes)
  • Photometric methods -> different absorption of light by certain amino acids
  • UV-vis, circular dichromism, and fluorescence spectroscopy -> different absorptions of electromagnetic radiation by the different AA
  • Mass spectrometry
Lösung ausblenden
TESTE DEIN WISSEN

How is the property of circular dichroism used in protein analysis?

Why does this work?

Lösung anzeigen
TESTE DEIN WISSEN

It is used to give hints to the identity of a protein. 

-> As left and right circularly polarized light superpositioned gives linerarly polarized light and a protein effects the 2 components differently, linearly polarized light can be used to shine it through a solution of the protein, observing how the polarization of the light tilts to one or the other position, letting the observer make inferences about the protein identity.

Lösung ausblenden
TESTE DEIN WISSEN

What do peroxidase-catalases have in common?

Lösung anzeigen
TESTE DEIN WISSEN

They share a similar active site structure

Lösung ausblenden
TESTE DEIN WISSEN

What 2 enzymes is the KarG very similar to?

Lösung anzeigen
TESTE DEIN WISSEN

Ascorbate peroxidase

Cytochrome C peroxidase

Lösung ausblenden
TESTE DEIN WISSEN

At what point is Vmax in an enzyme-substrate mixture reached?

Lösung anzeigen
TESTE DEIN WISSEN

It is reached, when the addition of more substrate to the mix will not increase the reaction rate Why -> all the active sites will be blocked in this moment

Lösung ausblenden
TESTE DEIN WISSEN

give a definition of km!

Lösung anzeigen
TESTE DEIN WISSEN

It is the concentration of substrate, at which the reaction rate of the enzyme is half of the maximum achievable

Lösung ausblenden
TESTE DEIN WISSEN

What is kcat?

Lösung anzeigen
TESTE DEIN WISSEN

it is the number of reactions, a single enzyme protein can catalyze in a second

Lösung ausblenden
TESTE DEIN WISSEN

What group am I in?

Lösung anzeigen
TESTE DEIN WISSEN

Group 3

Lösung ausblenden
TESTE DEIN WISSEN

When is an electric wave polarized?

Lösung anzeigen
TESTE DEIN WISSEN

When the electric field oscillates only in a linear plane.

Lösung ausblenden
TESTE DEIN WISSEN

What are Far-UV spectra in CD good for?

What absorbs here? What wavelength exactly?

Lösung anzeigen
TESTE DEIN WISSEN

-> investigating the secondary structure of the protein 

-> the peptide bond absorbs here -> at around 200nm

Lösung ausblenden
  • 25837 Karteikarten
  • 817 Studierende
  • 19 Lernmaterialien

Beispielhafte Karteikarten für deinen Biochemistry Kurs an der Universität für Bodenkultur Wien - von Kommilitonen auf StudySmarter erstellt!

Q:

Name a few prosthetic groups


A:
  • Flavins
  • Heme groups
  • Chlorophylls
Q:

What is the name of our enzyme version?

A:

D152S

Q:

What kinds of methods are used to investigate the enzyme?

A:
  • Electrophoretic methods (divide by charges -> different charges on protein and different sizes)
  • Photometric methods -> different absorption of light by certain amino acids
  • UV-vis, circular dichromism, and fluorescence spectroscopy -> different absorptions of electromagnetic radiation by the different AA
  • Mass spectrometry
Q:

How is the property of circular dichroism used in protein analysis?

Why does this work?

A:

It is used to give hints to the identity of a protein. 

-> As left and right circularly polarized light superpositioned gives linerarly polarized light and a protein effects the 2 components differently, linearly polarized light can be used to shine it through a solution of the protein, observing how the polarization of the light tilts to one or the other position, letting the observer make inferences about the protein identity.

Q:

What do peroxidase-catalases have in common?

A:

They share a similar active site structure

Mehr Karteikarten anzeigen
Q:

What 2 enzymes is the KarG very similar to?

A:

Ascorbate peroxidase

Cytochrome C peroxidase

Q:

At what point is Vmax in an enzyme-substrate mixture reached?

A:

It is reached, when the addition of more substrate to the mix will not increase the reaction rate Why -> all the active sites will be blocked in this moment

Q:

give a definition of km!

A:

It is the concentration of substrate, at which the reaction rate of the enzyme is half of the maximum achievable

Q:

What is kcat?

A:

it is the number of reactions, a single enzyme protein can catalyze in a second

Q:

What group am I in?

A:

Group 3

Q:

When is an electric wave polarized?

A:

When the electric field oscillates only in a linear plane.

Q:

What are Far-UV spectra in CD good for?

What absorbs here? What wavelength exactly?

A:

-> investigating the secondary structure of the protein 

-> the peptide bond absorbs here -> at around 200nm

Biochemistry

Erstelle und finde Lernmaterialien auf StudySmarter.

Greife kostenlos auf tausende geteilte Karteikarten, Zusammenfassungen, Altklausuren und mehr zu.

Jetzt loslegen

Die all-in-one Lernapp für Studierende

Greife auf Millionen geteilter Lernmaterialien der StudySmarter Community zu
Kostenlos anmelden Biochemistry
Erstelle Karteikarten und Zusammenfassungen mit den StudySmarter Tools
Kostenlos loslegen Biochemistry