Tet1 an der Universität Duisburg-Essen | Karteikarten & Zusammenfassungen

Lernmaterialien für Tet1 an der Universität Duisburg-Essen

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TESTE DEIN WISSEN
For which range of wavelenght measurements are quartz cuvettes useful?
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TESTE DEIN WISSEN
> 200 nm
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TESTE DEIN WISSEN
Name the formula used for the quantification of protein by UV/VIS spectroscopy in the presence of nucleic acids.
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TESTE DEIN WISSEN
Protein quantification by Warburg/Christian: Protein concentration = (1.55 * A280) - (0.76 * A260)
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TESTE DEIN WISSEN
On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?
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TESTE DEIN WISSEN
The energy of UV/VIS light is large enough to promote the transition of valence electrons to higher energy levels. Hence, the molecule reaches an excited electronic state for a short time interval,
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TESTE DEIN WISSEN
Name and explain the formula which can be used to calculate the energy of light.
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TESTE DEIN WISSEN
E = h * v = (h * c) / λ E = energy of a photon [J] h = 6.626 * 10(^-34) [Js] (Planck's constant) v = frequency [s^-1] λ = wavelenght [nm] c = 3 * 10^8 m/s (speed of light)
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TESTE DEIN WISSEN
"The ... the wavelenght of light, the higher its energy."
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TESTE DEIN WISSEN
shorter
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TESTE DEIN WISSEN
What is the working range of UV/VIS spectroscopy?
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TESTE DEIN WISSEN
200 - 800 nm
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TESTE DEIN WISSEN
Which wavelenghts give UV-C light?
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TESTE DEIN WISSEN
200 - 280 nm
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TESTE DEIN WISSEN
Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?
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TESTE DEIN WISSEN
Below 200 nm (mostly starting at 190 nm), the UV absorption of oxygen becomes significant and interferes with the measurement.
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TESTE DEIN WISSEN
Which wavelenghts give UV-B light?
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TESTE DEIN WISSEN
280 - 320 nm
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TESTE DEIN WISSEN
Which wavelenghts give UV-A light?
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TESTE DEIN WISSEN
320 - 400 nm
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TESTE DEIN WISSEN
Which wavelenghts give UV-A light?
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TESTE DEIN WISSEN
320 - 400 nm
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TESTE DEIN WISSEN
How much energy does a molecule absorb from the UV/VIS light?
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TESTE DEIN WISSEN
The energy of the absorbed light must equal the energy difference E between state 1 and 2.
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Q:
For which range of wavelenght measurements are quartz cuvettes useful?
A:
> 200 nm
Q:
Name the formula used for the quantification of protein by UV/VIS spectroscopy in the presence of nucleic acids.
A:
Protein quantification by Warburg/Christian: Protein concentration = (1.55 * A280) - (0.76 * A260)
Q:
On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?
A:
The energy of UV/VIS light is large enough to promote the transition of valence electrons to higher energy levels. Hence, the molecule reaches an excited electronic state for a short time interval,
Q:
Name and explain the formula which can be used to calculate the energy of light.
A:
E = h * v = (h * c) / λ E = energy of a photon [J] h = 6.626 * 10(^-34) [Js] (Planck's constant) v = frequency [s^-1] λ = wavelenght [nm] c = 3 * 10^8 m/s (speed of light)
Q:
"The ... the wavelenght of light, the higher its energy."
A:
shorter
Q:
What is the working range of UV/VIS spectroscopy?
A:
200 - 800 nm
Q:
Which wavelenghts give UV-C light?
A:
200 - 280 nm
Q:
Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?
A:
Below 200 nm (mostly starting at 190 nm), the UV absorption of oxygen becomes significant and interferes with the measurement.
Q:
Which wavelenghts give UV-B light?
A:
280 - 320 nm
Q:
Which wavelenghts give UV-A light?
A:
320 - 400 nm
Q:
Which wavelenghts give UV-A light?
A:
320 - 400 nm
Q:
How much energy does a molecule absorb from the UV/VIS light?
A:
The energy of the absorbed light must equal the energy difference E between state 1 and 2.

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