Introduction To Biological Imaging an der TU München | Karteikarten & Zusammenfassungen

# Lernmaterialien für Introduction to Biological Imaging an der TU München

Greife auf kostenlose Karteikarten, Zusammenfassungen, Übungsaufgaben und Altklausuren für deinen Introduction to Biological Imaging Kurs an der TU München zu.

TESTE DEIN WISSEN

What are the advantages of FDM?

Lösung anzeigen
TESTE DEIN WISSEN

1. Based on the differential form - no mathematical tinkering is necessary!

2. Simple implementation

3. Works well for simple geometries

Lösung ausblenden
TESTE DEIN WISSEN

Summary/Steps of FDM

Lösung anzeigen
TESTE DEIN WISSEN
1.  Use the differential formulation of the PDE

2.  Discretize the domain by selecting a set of grid points

3. Approximate the differential operator by suitable algebraic differences

4. Build a matrix for the differential equation with the given discretization.

Lösung ausblenden
TESTE DEIN WISSEN

Biopsy Definition/Steps

Lösung anzeigen
TESTE DEIN WISSEN

Taking pieces of tissue → slicing it very thin slices around 10µm → staining it → putting it under the microscope

Lösung ausblenden
TESTE DEIN WISSEN

Lösung anzeigen
TESTE DEIN WISSEN

1. Not so simple implementation

2. Still no energy conservation

Lösung ausblenden
TESTE DEIN WISSEN

Example of bioluminescence imaging

Lösung anzeigen
TESTE DEIN WISSEN

injected luc labeled neural progenitor cells migrate across the brain midline attracted by a contralaterally implanted glioma (tumor).

Lösung ausblenden
TESTE DEIN WISSEN

What are the ranges of resolution and imaging depth in microscopy?

Lösung anzeigen
TESTE DEIN WISSEN
• Resolution: 0,1µm to 1µm (of microscopes: around 0,5µm

• Imaging depth: 0,1mm to 10cm

Lösung ausblenden
TESTE DEIN WISSEN

Resolution depending on the propagation distance under the effect of scarttering

Lösung anzeigen
TESTE DEIN WISSEN
• 0-0,1mm: MFT (Mean free path), all photons experience at least one scattering event

• 0,1-1,0mm: increasing photon-scattering

• >1,0mm: TMFT (transport mean free path), random walk

Lösung ausblenden
TESTE DEIN WISSEN

Scattering components (in biological tissue)

Lösung anzeigen
TESTE DEIN WISSEN

* reflection by the plasma multilayer

* scattering by mitochondria (1µm, the same size as bacteria)

Lösung ausblenden
TESTE DEIN WISSEN

Ways to use fluorescence in microscopy

Lösung anzeigen
TESTE DEIN WISSEN

a. Nonspecific probes: detect physiology (blood volume, angiogenesis)

b. Targeted probes: used to localize proteins and determine structure

c. Activatable 'smart' sensor probes: used to localize enzymes and determine the function

Lösung ausblenden
TESTE DEIN WISSEN

How to label cells with fluorescence?

Lösung anzeigen
TESTE DEIN WISSEN

Fluorescent proteins are responsible with their ribosomes for the fluorescence → labeling the cells by integrating the proteins in the samples

Lösung ausblenden
TESTE DEIN WISSEN

Lösung anzeigen
TESTE DEIN WISSEN

1. Handling curved boundaries is problematic

2. Difficult to handle material discontinuities

3. Grid refinement is not straightforward

4. No energy conservation

5. Cannot handle non-smooth terms

Lösung ausblenden
TESTE DEIN WISSEN

Lösung anzeigen
TESTE DEIN WISSEN

1. Works well for curved boundaries geometries

2. Can handle non-smoothness

5. Handles material discontinuities well

Lösung ausblenden
• 339440 Karteikarten
• 7678 Studierende
• 332 Lernmaterialien

## Beispielhafte Karteikarten für deinen Introduction to Biological Imaging Kurs an der TU München - von Kommilitonen auf StudySmarter erstellt!

Q:

What are the advantages of FDM?

A:

1. Based on the differential form - no mathematical tinkering is necessary!

2. Simple implementation

3. Works well for simple geometries

Q:

Summary/Steps of FDM

A:
1.  Use the differential formulation of the PDE

2.  Discretize the domain by selecting a set of grid points

3. Approximate the differential operator by suitable algebraic differences

4. Build a matrix for the differential equation with the given discretization.

Q:

Biopsy Definition/Steps

A:

Taking pieces of tissue → slicing it very thin slices around 10µm → staining it → putting it under the microscope

Q:

A:

1. Not so simple implementation

2. Still no energy conservation

Q:

Example of bioluminescence imaging

A:

injected luc labeled neural progenitor cells migrate across the brain midline attracted by a contralaterally implanted glioma (tumor).

Q:

What are the ranges of resolution and imaging depth in microscopy?

A:
• Resolution: 0,1µm to 1µm (of microscopes: around 0,5µm

• Imaging depth: 0,1mm to 10cm

Q:

Resolution depending on the propagation distance under the effect of scarttering

A:
• 0-0,1mm: MFT (Mean free path), all photons experience at least one scattering event

• 0,1-1,0mm: increasing photon-scattering

• >1,0mm: TMFT (transport mean free path), random walk

Q:

Scattering components (in biological tissue)

A:

* reflection by the plasma multilayer

* scattering by mitochondria (1µm, the same size as bacteria)

Q:

Ways to use fluorescence in microscopy

A:

a. Nonspecific probes: detect physiology (blood volume, angiogenesis)

b. Targeted probes: used to localize proteins and determine structure

c. Activatable 'smart' sensor probes: used to localize enzymes and determine the function

Q:

How to label cells with fluorescence?

A:

Fluorescent proteins are responsible with their ribosomes for the fluorescence → labeling the cells by integrating the proteins in the samples

Q:

A:

1. Handling curved boundaries is problematic

2. Difficult to handle material discontinuities

3. Grid refinement is not straightforward

4. No energy conservation

5. Cannot handle non-smooth terms

Q:

A:

1. Works well for curved boundaries geometries

2. Can handle non-smoothness

5. Handles material discontinuities well

### Erstelle und finde Lernmaterialien auf StudySmarter.

Greife kostenlos auf tausende geteilte Karteikarten, Zusammenfassungen, Altklausuren und mehr zu.

## Das sind die beliebtesten StudySmarter Kurse für deinen Studiengang Introduction to Biological Imaging an der TU München

Für deinen Studiengang Introduction to Biological Imaging an der TU München gibt es bereits viele Kurse, die von deinen Kommilitonen auf StudySmarter erstellt wurden. Karteikarten, Zusammenfassungen, Altklausuren, Übungsaufgaben und mehr warten auf dich!