Introduction To Biological Imaging an der TU München | Karteikarten & Zusammenfassungen

Lernmaterialien für Introduction to Biological Imaging an der TU München

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What are the advantages of FDM?

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1. Based on the differential form - no mathematical tinkering is necessary!

2. Simple implementation

3. Works well for simple geometries

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Summary/Steps of FDM

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  1.  Use the differential formulation of the PDE

  2.  Discretize the domain by selecting a set of grid points

  3. Approximate the differential operator by suitable algebraic differences

  4. Build a matrix for the differential equation with the given discretization.

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Biopsy Definition/Steps

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Taking pieces of tissue → slicing it very thin slices around 10µm → staining it → putting it under the microscope

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FEM Disadvantages

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1. Not so simple implementation

2. Still no energy conservation

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Example of bioluminescence imaging

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injected luc labeled neural progenitor cells migrate across the brain midline attracted by a contralaterally implanted glioma (tumor).

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What are the ranges of resolution and imaging depth in microscopy?

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  • Resolution: 0,1µm to 1µm (of microscopes: around 0,5µm

  • Imaging depth: 0,1mm to 10cm

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Resolution depending on the propagation distance under the effect of scarttering

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    • 0-0,1mm: MFT (Mean free path), all photons experience at least one scattering event

    • 0,1-1,0mm: increasing photon-scattering

    • >1,0mm: TMFT (transport mean free path), random walk

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Scattering components (in biological tissue)

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* reflection by the plasma multilayer

* scattering by mitochondria (1µm, the same size as bacteria)

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Ways to use fluorescence in microscopy

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a. Nonspecific probes: detect physiology (blood volume, angiogenesis)

b. Targeted probes: used to localize proteins and determine structure

c. Activatable 'smart' sensor probes: used to localize enzymes and determine the function

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How to label cells with fluorescence?

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Fluorescent proteins are responsible with their ribosomes for the fluorescence → labeling the cells by integrating the proteins in the samples

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Disadvantages FDM

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1. Handling curved boundaries is problematic

2. Difficult to handle material discontinuities

3. Grid refinement is not straightforward

4. No energy conservation

5. Cannot handle non-smooth terms

Lösung ausblenden
TESTE DEIN WISSEN

FEM Advantages

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1. Works well for curved boundaries geometries

2. Can handle non-smoothness

3. H-adaptivity

4. P-adaptivity

5. Handles material discontinuities well

Lösung ausblenden
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Q:

What are the advantages of FDM?

A:

1. Based on the differential form - no mathematical tinkering is necessary!

2. Simple implementation

3. Works well for simple geometries

Q:

Summary/Steps of FDM

A:
  1.  Use the differential formulation of the PDE

  2.  Discretize the domain by selecting a set of grid points

  3. Approximate the differential operator by suitable algebraic differences

  4. Build a matrix for the differential equation with the given discretization.

Q:

Biopsy Definition/Steps

A:

Taking pieces of tissue → slicing it very thin slices around 10µm → staining it → putting it under the microscope

Q:

FEM Disadvantages

A:

1. Not so simple implementation

2. Still no energy conservation

Q:

Example of bioluminescence imaging

A:

injected luc labeled neural progenitor cells migrate across the brain midline attracted by a contralaterally implanted glioma (tumor).

Mehr Karteikarten anzeigen
Q:

What are the ranges of resolution and imaging depth in microscopy?

A:
  • Resolution: 0,1µm to 1µm (of microscopes: around 0,5µm

  • Imaging depth: 0,1mm to 10cm

Q:

Resolution depending on the propagation distance under the effect of scarttering

A:
    • 0-0,1mm: MFT (Mean free path), all photons experience at least one scattering event

    • 0,1-1,0mm: increasing photon-scattering

    • >1,0mm: TMFT (transport mean free path), random walk

Q:

Scattering components (in biological tissue)

A:

* reflection by the plasma multilayer

* scattering by mitochondria (1µm, the same size as bacteria)

Q:

Ways to use fluorescence in microscopy

A:

a. Nonspecific probes: detect physiology (blood volume, angiogenesis)

b. Targeted probes: used to localize proteins and determine structure

c. Activatable 'smart' sensor probes: used to localize enzymes and determine the function

Q:

How to label cells with fluorescence?

A:

Fluorescent proteins are responsible with their ribosomes for the fluorescence → labeling the cells by integrating the proteins in the samples

Q:

Disadvantages FDM

A:

1. Handling curved boundaries is problematic

2. Difficult to handle material discontinuities

3. Grid refinement is not straightforward

4. No energy conservation

5. Cannot handle non-smooth terms

Q:

FEM Advantages

A:

1. Works well for curved boundaries geometries

2. Can handle non-smoothness

3. H-adaptivity

4. P-adaptivity

5. Handles material discontinuities well

Introduction to Biological Imaging

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