Microbiology an der Hochschule Bonn-Rhein-Sieg | Karteikarten & Zusammenfassungen

Lernmaterialien für Microbiology an der Hochschule Bonn-Rhein-Sieg

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Rhibozobium

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Gram -
Habitat soil
Oxygen obligate aerobe
Shape rod
Fixes gaseous nitrogen in nodules on plant roots, doesn’t fix unless it’s in symbiotic relationship with a plant , responsible for production of Ammonia (NH4+) in nitrogencycle

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which mo is a better choice in the production of therapeutic proteins?

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yeast! 

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Bacillus thuringiesis + subtilis

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Gram +
Habitat soil
Oxygen -
Shape rod
Can form endoscopes, use as larvicide (Bt-toxin) Gene for toxin is integrated into plant DNA to make it produce toxin itself

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The length of the rod shaped organism such as Escherichia  coli is app.

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2 µm

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Mycobacterium (M. Tuberculosis + leprae)

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Gram thick cell wall, staining with acid-fast-stain
Habitat -
Oxygen -
Shape rod
Mycolic acid gives cell wall and colonies a waxy appearance and feel, protecting it from desiccation and disinfection, extraordinary thick wall doesn’t allow it to be stained

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Streptococcus mutants (strepto = chain of cells)

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Gram +
Habitat mouth, part of normal flora
Oxygen -
Shape coccus
Glycoproteins of saliva form film on tooth surface, s.mutans bindsand forms microcolonies as well as biofilms in the presence of sucrose. Heavy Growth plus lactic Acid fermentation leads to demateralization of the the teeth

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MOs can be used as producers for which recombinant proteins?

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  • Hormones (insulin!) 
  • Vitamins 
  • Cytokines 
  • Vaccines 
  • Modified therapeutic antibodies



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Micrococcus luteus

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Gram +
Habitat: Skin, dust, Soil
Oxygen:/
While diving, cells don’t properly separate, forming clusters of two or 4 cells called tetrads but no chains

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Escherichia coli

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Gram -
Habitat intestine, part of normal flora
Oxygen -
Shape rod
Entirely sequenced, some belong to normal flora, but some can be pathogenic, genome easily manipulated for our benefit and typical microbe for cultivation due to fast generation time, best known enterobacteria (colonize intestine) and is distinguishable by fermentation -> endoagar

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Ruminant animals rely on microorganisms for

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fixing N2 from air



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Scientific nomenclature is binominal:

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each organism is assigned two names: a genus and a specific epithet, or species

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particular DNA sequences, so-called : evolutionary chronometers:

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These are DNA-sequences which 

  • occur in all organisms which you want to compare 
  • contain conserved stretches
  • contain variable stretches 
  • Often used: 16 S/ 18 S rRNA
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  • 41636 Karteikarten
  • 813 Studierende
  • 32 Lernmaterialien

Beispielhafte Karteikarten für deinen Microbiology Kurs an der Hochschule Bonn-Rhein-Sieg - von Kommilitonen auf StudySmarter erstellt!

Q:

Rhibozobium

A:

Gram -
Habitat soil
Oxygen obligate aerobe
Shape rod
Fixes gaseous nitrogen in nodules on plant roots, doesn’t fix unless it’s in symbiotic relationship with a plant , responsible for production of Ammonia (NH4+) in nitrogencycle

Q:

which mo is a better choice in the production of therapeutic proteins?

A:

yeast! 

Q:

Bacillus thuringiesis + subtilis

A:

Gram +
Habitat soil
Oxygen -
Shape rod
Can form endoscopes, use as larvicide (Bt-toxin) Gene for toxin is integrated into plant DNA to make it produce toxin itself

Q:

The length of the rod shaped organism such as Escherichia  coli is app.

A:

2 µm

Q:

Mycobacterium (M. Tuberculosis + leprae)

A:

Gram thick cell wall, staining with acid-fast-stain
Habitat -
Oxygen -
Shape rod
Mycolic acid gives cell wall and colonies a waxy appearance and feel, protecting it from desiccation and disinfection, extraordinary thick wall doesn’t allow it to be stained

Mehr Karteikarten anzeigen
Q:

Streptococcus mutants (strepto = chain of cells)

A:

Gram +
Habitat mouth, part of normal flora
Oxygen -
Shape coccus
Glycoproteins of saliva form film on tooth surface, s.mutans bindsand forms microcolonies as well as biofilms in the presence of sucrose. Heavy Growth plus lactic Acid fermentation leads to demateralization of the the teeth

Q:

MOs can be used as producers for which recombinant proteins?

A:
  • Hormones (insulin!) 
  • Vitamins 
  • Cytokines 
  • Vaccines 
  • Modified therapeutic antibodies



Q:

Micrococcus luteus

A:

Gram +
Habitat: Skin, dust, Soil
Oxygen:/
While diving, cells don’t properly separate, forming clusters of two or 4 cells called tetrads but no chains

Q:

Escherichia coli

A:

Gram -
Habitat intestine, part of normal flora
Oxygen -
Shape rod
Entirely sequenced, some belong to normal flora, but some can be pathogenic, genome easily manipulated for our benefit and typical microbe for cultivation due to fast generation time, best known enterobacteria (colonize intestine) and is distinguishable by fermentation -> endoagar

Q:

Ruminant animals rely on microorganisms for

A:



fixing N2 from air



Q:

Scientific nomenclature is binominal:

A:

each organism is assigned two names: a genus and a specific epithet, or species

Q:

particular DNA sequences, so-called : evolutionary chronometers:

A:

These are DNA-sequences which 

  • occur in all organisms which you want to compare 
  • contain conserved stretches
  • contain variable stretches 
  • Often used: 16 S/ 18 S rRNA
Microbiology

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