Kommilitonen im Kurs Genetic Engineering an der Hochschule Bonn-Rhein-Sieg. erstellen und teilen Zusammenfassungen, Karteikarten, Lernpläne und andere Lernmaterialien mit der intelligenten StudySmarter Lernapp. Jetzt mitmachen!
Jetzt mitmachen!
Genetic Engineering
Define "Recombinant DNA".
Any artificially produced DNA-molecule which brings together DNA sequences that are not usually found together in nature.
Genetic Engineering
Define "Genetic Engineering"
Techniques and procedures that are necessary to produce recombinant DNA and to transfer it into living cells.
Genetic Engineering
When did Genetic Engineering start?
In 1972.
Genetic Engineering
What is direct evolution and which steps are involved?
Direct evolution is a speed up evolution in a test tube and involves the following steps:
1. DNA isolation
2. Introduction of random mutagenesis
3. Repeat 1 and 2
4. Transfer back to organism
5. Analysis
Genetic Engineering
What where earlier techniques for introduction of random mutagenesis and how are we doing it nowadays?
Earlier, UV light was used for dimer formation.
Today, we use error-prone PCR.
Genetic Engineering
What is molecular cloning?
generation and propagation of recombinant DNA.
Genetic Engineering
Which steps are involved in molecular cloning?
1. DNA isolation of vector and insert (gene of interest)
2. Process DNA with restriction enzymes
3. Ligation
4. Transformation of host cell
5. Selection and Screening
Genetic Engineering
When is a organism genetically modified?
When recombinant DNA was introduced into it.
Genetic Engineering
When and for what can GMOs be used?
- to produce useful recombinant molecules
- to improve agronomic traits (by overexpression/repression of certain proteins or by showing new traits leading to e.g. herbicide resistance)
Genetic Engineering
In general, what are the advantages and disadvantages of microorganisms like E. coli as GMOs?
Advantages:
- genetics well understood
- unlimited quantities of protein generation possible
- fermentation technology well understood (faster growth, cheap medium)
Disadvantages:
- eukaryotic proteins cannot be folded correctly by prokaryot and might be inactive
- formation of inclusion bodies possible
Genetic Engineering
What are the challenges of using cell cultures as GMOs?
- Growth of cells (e.g. can they grow in fermenter?)
- Cell lines may have mutations and their extensiveness is not known (e.g. are they free of endogenous viruses?)
Genetic Engineering
What are transgenic animals?
Modified animals that can produce desired products.
Für deinen Studiengang Genetic Engineering an der Hochschule Bonn-Rhein-Sieg gibt es bereits viele Kurse auf StudySmarter, denen du beitreten kannst. Karteikarten, Zusammenfassungen und vieles mehr warten auf dich.
Zurück zur Hochschule Bonn-Rhein-Sieg ÜbersichtsseiteStudySmarter ist eine intelligente Lernapp für Studenten. Mit StudySmarter kannst du dir effizient und spielerisch Karteikarten, Zusammenfassungen, Mind-Maps, Lernpläne und mehr erstellen. Erstelle deine eigenen Karteikarten z.B. für Genetic Engineering an der Hochschule Bonn-Rhein-Sieg oder greife auf tausende Lernmaterialien deiner Kommilitonen zu. Egal, ob an deiner Uni oder an anderen Universitäten. Hunderttausende Studierende bereiten sich mit StudySmarter effizient auf ihre Klausuren vor. Erhältlich auf Web, Android & iOS. Komplett kostenfrei. Keine Haken.
Bestes EdTech Startup in Deutschland
Du bist schon registriert? Hier geht‘s zum Login