Microscopy at University Of Witwatersrand | Flashcards & Summaries

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Lernmaterialien für Microscopy an der University of Witwatersrand

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WHAT ARE THE 2 PROPERTIES OF A MICROSCOPE 

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-resolving power (r.p.)- the smallest separation at which we can see that 2 objects are present rather than 1 

-magnifying power- how many times the apparent size of the object has been increased

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WHAT ARE THE 2 TYPES OF LIGHT MICROSCOPES 

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  • stereo binocular 

    • light reflected off of specimen or through

    • used for dissection

    • can show gross morphology of species 

  • high power

    • light transmitted through species

    • used to examine tissues and some cellular detail

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SCANNING ELECTRON MICROSCOPE

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  • electron beam scans surface of specimen 

  • allows for detailed examination of surface structures 

  • looks 3d

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TRANSMISSION ELECTRON MICROSCOPE

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    • electron beams transmitted through specimen 

    • examination of very small structures eg viruses and organelles

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WHAT ARE THE MAIN UNITS OF MEASUREMENT 

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    • millimeter- mm 1x10-3m

    • micrometer- μm 1x10-6m

    • nanometer-nm 1x10-9m

    • picometer- pm 1x10-12m

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HOW IS THE VISUAL IMAGE FORMED IN A LM

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  • intermediate image reversed

  • retina image flipped again

  • brain flips image when processing

  • virtual image is reversed of actual specimen

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WHAT DOES RESOLVING POWER DEPEND ON 

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  • numerical aperture of the objective lens 

  • wave length of the radiation used to form the image 

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HOW IS RESOLVING POWER OF A LM CALCULATED 

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0.61λ/N.A.

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WHAT FACTORS AFFECT RESOLVING POWER

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  • aberrations in the lens used 

  • specimen thickness

  • specimen contrast 

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WHAT ARE THE 3 TYPES OF LENS ABERRATIONS

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  • chromatic

    • rays of different wavelengths from a point in the specimen focus at different distances resulting in an unsharp image 

  • spherical

    • rays from different points in the specimen that pass through diff regions of the lens, focus at diff distances resulting in an unsharp image 

  • field curvature 

    • a flat specimen is shown as curved so the centre and edges cant be focused simultaneously 

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WHAT FUNCTION DOES THE COVERSLIP SERVE

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  • light rays refracted at upper surface 

  • high power objectives designed to correct this refraction

  • w/out cover slip, the objective is under corrected -spherical aberration

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WHAT IS THE USE OF A MICROSCOPE 

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microscopes are used to render visible objects that are too small to be seen by the naked eye

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  • 40361 Karteikarten
  • 3454 Studierende
  • 33 Lernmaterialien

Beispielhafte Karteikarten für deinen Microscopy Kurs an der University of Witwatersrand - von Kommilitonen auf StudySmarter erstellt!

Q:

WHAT ARE THE 2 PROPERTIES OF A MICROSCOPE 

A:

-resolving power (r.p.)- the smallest separation at which we can see that 2 objects are present rather than 1 

-magnifying power- how many times the apparent size of the object has been increased

Q:

WHAT ARE THE 2 TYPES OF LIGHT MICROSCOPES 

A:
  • stereo binocular 

    • light reflected off of specimen or through

    • used for dissection

    • can show gross morphology of species 

  • high power

    • light transmitted through species

    • used to examine tissues and some cellular detail

Q:

SCANNING ELECTRON MICROSCOPE

A:
  • electron beam scans surface of specimen 

  • allows for detailed examination of surface structures 

  • looks 3d

Q:

TRANSMISSION ELECTRON MICROSCOPE

A:
    • electron beams transmitted through specimen 

    • examination of very small structures eg viruses and organelles

Q:

WHAT ARE THE MAIN UNITS OF MEASUREMENT 

A:
    • millimeter- mm 1x10-3m

    • micrometer- μm 1x10-6m

    • nanometer-nm 1x10-9m

    • picometer- pm 1x10-12m

Mehr Karteikarten anzeigen
Q:

HOW IS THE VISUAL IMAGE FORMED IN A LM

A:
  • intermediate image reversed

  • retina image flipped again

  • brain flips image when processing

  • virtual image is reversed of actual specimen

Q:

WHAT DOES RESOLVING POWER DEPEND ON 

A:
  • numerical aperture of the objective lens 

  • wave length of the radiation used to form the image 

Q:

HOW IS RESOLVING POWER OF A LM CALCULATED 

A:

0.61λ/N.A.

Q:

WHAT FACTORS AFFECT RESOLVING POWER

A:
  • aberrations in the lens used 

  • specimen thickness

  • specimen contrast 

Q:

WHAT ARE THE 3 TYPES OF LENS ABERRATIONS

A:
  • chromatic

    • rays of different wavelengths from a point in the specimen focus at different distances resulting in an unsharp image 

  • spherical

    • rays from different points in the specimen that pass through diff regions of the lens, focus at diff distances resulting in an unsharp image 

  • field curvature 

    • a flat specimen is shown as curved so the centre and edges cant be focused simultaneously 

Q:

WHAT FUNCTION DOES THE COVERSLIP SERVE

A:
  • light rays refracted at upper surface 

  • high power objectives designed to correct this refraction

  • w/out cover slip, the objective is under corrected -spherical aberration

Q:

WHAT IS THE USE OF A MICROSCOPE 

A:

microscopes are used to render visible objects that are too small to be seen by the naked eye

Microscopy

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