Greife auf kostenlose Karteikarten, Zusammenfassungen, Übungsaufgaben und Altklausuren für deinen Biopolymer Chemistry Kurs an der University of Tromsø zu.
PCR reaction mix
a) Draw the two nucleobase pairs present in DNA and b) use this drawing to show how the sequence of a DNA duplex can be read without opening the duplex through the major and minor grooves.
Draw the complete chemical structure of the oligonucleotide pTGCAp, using dash/
wedge notation to indicate stereochemistry.
Explain what Grove-reading is and How it works.
Reading of the DNA by Transcription factors+ polymerases. Specificity is caused by Sequence-recognition by the transcription factors: Bind the major-groove.
What are the 2 main factors that contribute to structural stability of DNA?
Hydrogen bonds between bp and π stacking of aromatic rings.
Why are there differences in directionality between chemical and biological DNA synthesis?
Biol: 5' ->3'
Pyrophosphate as leaving group is the driving force
Explain NGS Next gen sequencing
Many thousands of oligos on one chip, i don't really get it
PCR reaction mix
What are Glycoconjugates?
Strategies for protection of the prim. OH (6/6' OH) in pyranoses
Sterically demanding PG
Draw a Watson–Crick base pair between adenine and a thymidine with an R group at
the 5-position of the base. In which groove of DNA, major or minor, does the R group
What is triplex Binding? Where is it involved?
Recognition of DNA
Hoogsteen base pairing (vs Watson crick bp) can lead to triple stranded DNA by selective binding of SS-oligos to major groove of ds DNA.
Greife kostenlos auf tausende geteilte Karteikarten, Zusammenfassungen, Altklausuren und mehr zu.Jetzt loslegen