The Cell at University Of The Philippines Manila | Flashcards & Summaries

Lernmaterialien für The Cell an der University of the Philippines Manila

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This is known as the process by which the nucleus of the parent cell divides into two daughter nuclei.

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Karyokinesis

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Trace the production of a trypsin, a digestive enzyme produced by the pancreas, through the cellular organelles beginning with the trypsin gene in the nucleus and ending with its release into the lumen of the pancreatic duct.

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Nucleus, ribosome, RER, Golgi, secretory vesicle, pancreatic duct.

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What is the diameter of a human ovum and can we see it without a microscope?

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Yes, we can only see exceptionally large cells like the human ovum, which has a diameter of 100 µm.

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How might you design an experiment to trace the steps in the transport and processing of a lysosomal hydrolase? What would you expect your results to be? How does this differ from the results you would expect for a similar experiment designed to trace the steps in the processing of a protein component of the ribosome?

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Whatever experiment you design, you would expect the lysosomal hydrolase to be translated on the RER and then enter the Golgi and lysosome. A ribosomal protein would be translated in the cytoplasmic ribosomes, transported to the nucleus for assembly into a ribosome, then transported out of the nucleus back to the cytoplasm

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Explain what color you expect the RER and the SER to stain under H&E. If there is a difference, why?

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RER should be basophilic because of the presence of ribosomes (purplish), whereas SER should appear more eosinophilic. The lack of ribosomes means that the smooth ER is not basophilic and usually stains poorly by H&E.


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It is used as a stain for lipids and Golgi apparatus under a light microscope and as a fixative for electron microscopy.

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Osmium staining


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If you want to see smaller organelles like ribosomes, macromolecular assemblies, and macromolecules, what microscope will you use?


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Electron Microscope

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These are assemblies of specific macromolecules organized to carry out complex functions in the cell.

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Organelles

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It is gel-like substance/aqueous component of the cytoplasm containing dissolved macromolecules, organic compounds, ions and cytoskeleton.


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Cytosol

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What are the 3 parts of a cytoplasm?

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organelles, cytosol and inclusions 


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It is a blue dye used in H&E staining that is basic (positively charged) and binds to negatively charged DNA and RNA.

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Hematoxylin

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Place the following in order of increasing size: bacterium, nucleus, secretory granule, red blood cell, human ovum, eukaryotic cell, thickness of plasma membrane.

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Plasma membrane, secretory granule, nucleus, bacterium, red blood cell, eukaryotic cell, human ovum.

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Beispielhafte Karteikarten für deinen The Cell Kurs an der University of the Philippines Manila - von Kommilitonen auf StudySmarter erstellt!

Q:

This is known as the process by which the nucleus of the parent cell divides into two daughter nuclei.

A:

Karyokinesis

Q:

Trace the production of a trypsin, a digestive enzyme produced by the pancreas, through the cellular organelles beginning with the trypsin gene in the nucleus and ending with its release into the lumen of the pancreatic duct.

A:

Nucleus, ribosome, RER, Golgi, secretory vesicle, pancreatic duct.

Q:

What is the diameter of a human ovum and can we see it without a microscope?

A:

Yes, we can only see exceptionally large cells like the human ovum, which has a diameter of 100 µm.

Q:

How might you design an experiment to trace the steps in the transport and processing of a lysosomal hydrolase? What would you expect your results to be? How does this differ from the results you would expect for a similar experiment designed to trace the steps in the processing of a protein component of the ribosome?

A:

Whatever experiment you design, you would expect the lysosomal hydrolase to be translated on the RER and then enter the Golgi and lysosome. A ribosomal protein would be translated in the cytoplasmic ribosomes, transported to the nucleus for assembly into a ribosome, then transported out of the nucleus back to the cytoplasm

Q:

Explain what color you expect the RER and the SER to stain under H&E. If there is a difference, why?

A:

RER should be basophilic because of the presence of ribosomes (purplish), whereas SER should appear more eosinophilic. The lack of ribosomes means that the smooth ER is not basophilic and usually stains poorly by H&E.


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Q:

It is used as a stain for lipids and Golgi apparatus under a light microscope and as a fixative for electron microscopy.

A:

Osmium staining


Q:

If you want to see smaller organelles like ribosomes, macromolecular assemblies, and macromolecules, what microscope will you use?


A:

Electron Microscope

Q:

These are assemblies of specific macromolecules organized to carry out complex functions in the cell.

A:

Organelles

Q:

It is gel-like substance/aqueous component of the cytoplasm containing dissolved macromolecules, organic compounds, ions and cytoskeleton.


A:

Cytosol

Q:

What are the 3 parts of a cytoplasm?

A:

organelles, cytosol and inclusions 


Q:

It is a blue dye used in H&E staining that is basic (positively charged) and binds to negatively charged DNA and RNA.

A:

Hematoxylin

Q:

Place the following in order of increasing size: bacterium, nucleus, secretory granule, red blood cell, human ovum, eukaryotic cell, thickness of plasma membrane.

A:

Plasma membrane, secretory granule, nucleus, bacterium, red blood cell, eukaryotic cell, human ovum.

The Cell

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