PCR Amplification at University Of The Immaculate Conception | Flashcards & Summaries

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Lernmaterialien für PCR amplification an der University of the Immaculate Conception

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What is the first amplification that Kary Millus did? 

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Short fragment of the E. coli plasmid, pBR322

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Which of the following do not belong to the molecular amplification technique?

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DNA recombinant amplification technique 

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Which of the following is true about performing PCR?

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Subject the reaction mix to an amplification program 

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Which of the following is not true about automation of PCR?

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The thermal cycler changes temperatures in a block or chamber holding the pipette

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Which of the following does signal amplification need? Select 2 answers. 

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bDNA

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This type of PCR modification has two or more sets of primers specific to different targets

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Multiplex PCR 

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Which of the following is true about target amplification?

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Amplification products detected by 2 oligonucleotide primers 

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Which of the following is not true about the PCR cycle and its temperature?

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Extension temperature optimized for individual polymerase (70-72C, 20 seconds)

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Which of the following is not true about primers?

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Primer design is the general aspect of PCR 

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He envisioned the idea of amplifying DNA in vitro in 1983 

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TESTE DEIN WISSEN

Kary Millus 

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Which of the following is true about the interpretation of the PCR results?

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The PCR product should be of the exact expected size 

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Structural analysis, DNA typing, disease detection, cloning, mutation analysis, detection of gene expression, and mapping are just some of the PCR applications.

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True 

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Beispielhafte Karteikarten für deinen PCR amplification Kurs an der University of the Immaculate Conception - von Kommilitonen auf StudySmarter erstellt!

Q:

What is the first amplification that Kary Millus did? 

A:

Short fragment of the E. coli plasmid, pBR322

Q:

Which of the following do not belong to the molecular amplification technique?

A:

DNA recombinant amplification technique 

Q:

Which of the following is true about performing PCR?

A:

Subject the reaction mix to an amplification program 

Q:

Which of the following is not true about automation of PCR?

A:

The thermal cycler changes temperatures in a block or chamber holding the pipette

Q:

Which of the following does signal amplification need? Select 2 answers. 

A:

bDNA

Mehr Karteikarten anzeigen
Q:

This type of PCR modification has two or more sets of primers specific to different targets

A:

Multiplex PCR 

Q:

Which of the following is true about target amplification?

A:

Amplification products detected by 2 oligonucleotide primers 

Q:

Which of the following is not true about the PCR cycle and its temperature?

A:

Extension temperature optimized for individual polymerase (70-72C, 20 seconds)

Q:

Which of the following is not true about primers?

A:

Primer design is the general aspect of PCR 

Q:

He envisioned the idea of amplifying DNA in vitro in 1983 

A:

Kary Millus 

Q:

Which of the following is true about the interpretation of the PCR results?

A:

The PCR product should be of the exact expected size 

Q:

Structural analysis, DNA typing, disease detection, cloning, mutation analysis, detection of gene expression, and mapping are just some of the PCR applications.

A:

True 

PCR amplification

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