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What is the first amplification that Kary Millus did?
Short fragment of the E. coli plasmid, pBR322
Which of the following do not belong to the molecular amplification technique?
DNA recombinant amplification technique
Which of the following is true about performing PCR?
Subject the reaction mix to an amplification program
Which of the following is not true about automation of PCR?
The thermal cycler changes temperatures in a block or chamber holding the pipette
Which of the following does signal amplification need? Select 2 answers.
bDNA
This type of PCR modification has two or more sets of primers specific to different targets
Multiplex PCR
Which of the following is true about target amplification?
Amplification products detected by 2 oligonucleotide primers
Which of the following is not true about the PCR cycle and its temperature?
Extension temperature optimized for individual polymerase (70-72C, 20 seconds)
Which of the following is not true about primers?
Primer design is the general aspect of PCR
He envisioned the idea of amplifying DNA in vitro in 1983
Kary Millus
Which of the following is true about the interpretation of the PCR results?
The PCR product should be of the exact expected size
Structural analysis, DNA typing, disease detection, cloning, mutation analysis, detection of gene expression, and mapping are just some of the PCR applications.
True
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