Hist-O at University Of Alberta | Flashcards & Summaries

Lernmaterialien für Hist-O an der University of Alberta

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TESTE DEIN WISSEN
What is Bouins Fixative composed of?
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TESTE DEIN WISSEN
Formalin 

Acetic Acid

Picric Acid
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TESTE DEIN WISSEN
What fixative is best at decalcification and fixation of small tissues?
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TESTE DEIN WISSEN
Bouins
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When would EtOH be used for fixation?
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TESTE DEIN WISSEN
When Uric acid demonstration is requested
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TESTE DEIN WISSEN
When would you use fresh/frozen tissue instead of fixative?
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TESTE DEIN WISSEN
For molecular diagnostics
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TESTE DEIN WISSEN
When would cold isotonic saline be used instead of fixative?
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TESTE DEIN WISSEN
For preservation of enzymes in tissues like kidney and muscle.
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TESTE DEIN WISSEN
What is the max transport time for tissue in cold isotonic saline?
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TESTE DEIN WISSEN
1hr
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What is Michel's medium used for?
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Non-fixative, used for preservation of antigenicity. Therefore, tissue subjected to immunofluorescent methods
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When is RPMI used?
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TESTE DEIN WISSEN
For growth and maintenance of lymphoid tissue
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TESTE DEIN WISSEN
What is used to transport suspected lymphoma tissue? What protocol does this follow?
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TESTE DEIN WISSEN
RPMI. This is part of the Lymphoma Protocol
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TESTE DEIN WISSEN
How many identifiers must be on a paraffin block?
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TESTE DEIN WISSEN
Only 1. The specimen number is the only requirement, but many labs have labels with more info now
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TESTE DEIN WISSEN
What must be done in the following situations?

Unlabelled specimen
Tissue Type not specified
Patients do not match bw specimen and req
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TESTE DEIN WISSEN
Hold testing until contact with physician is made.

Get them to fill out a waiver of responsibility before testing commences 
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TESTE DEIN WISSEN
What fixative should be used for preservation of PNS? What test is tissue fixed with this good for?
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TESTE DEIN WISSEN
Glutaraldehyde. Good for EM 
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Q:
What is Bouins Fixative composed of?
A:
Formalin 

Acetic Acid

Picric Acid
Q:
What fixative is best at decalcification and fixation of small tissues?
A:
Bouins
Q:
When would EtOH be used for fixation?
A:
When Uric acid demonstration is requested
Q:
When would you use fresh/frozen tissue instead of fixative?
A:
For molecular diagnostics
Q:
When would cold isotonic saline be used instead of fixative?
A:
For preservation of enzymes in tissues like kidney and muscle.
Mehr Karteikarten anzeigen
Q:
What is the max transport time for tissue in cold isotonic saline?
A:
1hr
Q:
What is Michel's medium used for?
A:
Non-fixative, used for preservation of antigenicity. Therefore, tissue subjected to immunofluorescent methods
Q:
When is RPMI used?
A:
For growth and maintenance of lymphoid tissue
Q:
What is used to transport suspected lymphoma tissue? What protocol does this follow?
A:
RPMI. This is part of the Lymphoma Protocol
Q:
How many identifiers must be on a paraffin block?
A:
Only 1. The specimen number is the only requirement, but many labs have labels with more info now
Q:
What must be done in the following situations?

Unlabelled specimen
Tissue Type not specified
Patients do not match bw specimen and req
A:
Hold testing until contact with physician is made.

Get them to fill out a waiver of responsibility before testing commences 
Q:
What fixative should be used for preservation of PNS? What test is tissue fixed with this good for?
A:
Glutaraldehyde. Good for EM 
Hist-O

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