Basic Virology at Universität Göttingen | Flashcards & Summaries

Lernmaterialien für Basic virology an der Universität Göttingen

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Under which experimental conditions will E. coli and influenza virus replicate: cells in culture oder luria broth medium?


             

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  - cell culture for viruses, medium for bacteria

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You need to find out whether a patient is infected by a virus. To do this, you only have a serum sample and a flask of Vero E6 African green monkey cells. How do you proceed?


               

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- Inoculate cells with serum sample and a control sample.

Assess whether cells develop CPE and if CPE increases over time

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Which of the two viruses (Adeno, Herpes) will likely be more sensitive to inactivation by disinfectants?


              

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 - Herpes, because it has a membrane -> more easily destroyed

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Which viral replication strategies can DNA viruses do?



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1. RNA priming (e.g. Polymonaviridae)

2. Protein priming (e.g. Adenoviridae)

3. Hairpin priming (e.g. Parvoviridae)

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How can you test a patient for a virus?


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ELISA, PCR, EM, inoculate cell cultures, IFT, neutralization test

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How do (+) ssRNA viruses achieve synthesis of several mature

polypeptides?

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no transcription required, (+) ssRNA already serves as mRNA

or

polyprotein processing

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How would you diagnose a successful HBV vaccination?



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- by measuring Anti-HBs levels

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What is cap snatching?

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First step of replication: —ssRNA Viruses


- mechanism that turns viral RNA into cellular mRNA lookalikes in order to make the endogenous cellular machinery carry

out processing and nuclear export

- first 10 to 20 bp of a host cell RNA are removed (snatched)               

- used as the 5′ cap and primer to initiate the synthesis of viral mRNA

—> polymerase can replicate — Sense genome from the + template
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How is viral DNA replication started? Name three priming mechanisms.

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RNA priming, protein priming, hairpin priming

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What are the three characteristics of an transcriptional enhancer?

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orientation independent, long distance, contains multiple sequence elements

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What's the difference between acute and latent infection?


               

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- Acute: Virus is cleared (or host dies)


- Latent: Special form of persistent infection, where virus replication continues despite                immune response (-> immune evasion!). During certain stages, replication cannot be                detected even though the virus is still present

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State major commonality between SARS-CoV1 and SARS-CoV2



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- Spike protein of both viruses use ACE2 as receptor for host cell entry

 

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Q:


Under which experimental conditions will E. coli and influenza virus replicate: cells in culture oder luria broth medium?


             

A:

  - cell culture for viruses, medium for bacteria

Q:


You need to find out whether a patient is infected by a virus. To do this, you only have a serum sample and a flask of Vero E6 African green monkey cells. How do you proceed?


               

A:

- Inoculate cells with serum sample and a control sample.

Assess whether cells develop CPE and if CPE increases over time

Q:


Which of the two viruses (Adeno, Herpes) will likely be more sensitive to inactivation by disinfectants?


              

A:

 - Herpes, because it has a membrane -> more easily destroyed

Q:


Which viral replication strategies can DNA viruses do?



A:

1. RNA priming (e.g. Polymonaviridae)

2. Protein priming (e.g. Adenoviridae)

3. Hairpin priming (e.g. Parvoviridae)

Q:

How can you test a patient for a virus?


A:

ELISA, PCR, EM, inoculate cell cultures, IFT, neutralization test

Mehr Karteikarten anzeigen
Q:

How do (+) ssRNA viruses achieve synthesis of several mature

polypeptides?

A:

no transcription required, (+) ssRNA already serves as mRNA

or

polyprotein processing

Q:


How would you diagnose a successful HBV vaccination?



A:

- by measuring Anti-HBs levels

Q:

What is cap snatching?

A:

First step of replication: —ssRNA Viruses


- mechanism that turns viral RNA into cellular mRNA lookalikes in order to make the endogenous cellular machinery carry

out processing and nuclear export

- first 10 to 20 bp of a host cell RNA are removed (snatched)               

- used as the 5′ cap and primer to initiate the synthesis of viral mRNA

—> polymerase can replicate — Sense genome from the + template
Q:

How is viral DNA replication started? Name three priming mechanisms.

A:

RNA priming, protein priming, hairpin priming

Q:

What are the three characteristics of an transcriptional enhancer?

A:

orientation independent, long distance, contains multiple sequence elements

Q:


What's the difference between acute and latent infection?


               

A:

- Acute: Virus is cleared (or host dies)


- Latent: Special form of persistent infection, where virus replication continues despite                immune response (-> immune evasion!). During certain stages, replication cannot be                detected even though the virus is still present

Q:


State major commonality between SARS-CoV1 and SARS-CoV2



A:

- Spike protein of both viruses use ACE2 as receptor for host cell entry

 

Basic virology

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