Select your language

Suggested languages for you:
Log In Start studying!

Lernmaterialien für Selected Topics in Biotechnology an der Universität für Bodenkultur Wien

Greife auf kostenlose Karteikarten, Zusammenfassungen, Übungsaufgaben und Altklausuren für deinen Selected Topics in Biotechnology Kurs an der Universität für Bodenkultur Wien zu.

TESTE DEIN WISSEN

Biotechnology (colors)

Lösung anzeigen
TESTE DEIN WISSEN
  • Green: agriculture, Plant BT 
  • White: Industry (industrial BT) 
  • Gray: waste management, environmental BT
  • Red: medical field, pharmaceutics, medical BT
  • Blue: exploitation of aquatic resources
Lösung ausblenden
TESTE DEIN WISSEN

What is red biotechnology and what are its typical products?

Lösung anzeigen
TESTE DEIN WISSEN

medical biotechnology 

e.g., antibodies, insulin, …

Lösung ausblenden
TESTE DEIN WISSEN

What is subtilisin and how is it produced?

Lösung anzeigen
TESTE DEIN WISSEN
  • Subtilisin are serin proteases from B. subtilis used as detergent enzymes
  • They are produced with a plasmid containing a gene for an antibiotic resistance
Lösung ausblenden
TESTE DEIN WISSEN

Describe the group of Biopolymers

Lösung anzeigen
TESTE DEIN WISSEN

Polymers consisting of biologically produced molecules 

  • carbohydrates (starch, etc.…)
  • nucleic acids (chain of N-bases) 
  • proteins (amino acid chains)
Lösung ausblenden
TESTE DEIN WISSEN

How is butanol and acetone produced biotechnologically?

Lösung anzeigen
TESTE DEIN WISSEN
  • Butanol and acetone can be produced by fermentation of anaerobic spore formers e.g. clostridia 
  • Corn is used as starting material and doesn´t need to be saccharified 
  • Fermentation runs strictly anaerobic under CO2 atmosphere
Lösung ausblenden
TESTE DEIN WISSEN

Describe the production of one red biotechnology product

Lösung anzeigen
TESTE DEIN WISSEN
  • insulin can be produced recombinantly in S. cerevisiae 
  • the protein is secreted and can be purified with lysing the cells 
  • insulin is expressed with a shorter C-peptide which is easily cleaved off after purification
Lösung ausblenden
TESTE DEIN WISSEN

What substrates are suitable for ethanol production? What technical difficulties have to be considered?

Lösung anzeigen
TESTE DEIN WISSEN

starch can be used as substrate, but it needs to be broken down by amylases and saccharified by glocoamylase

Lösung ausblenden
TESTE DEIN WISSEN

How is acetic acid produced ->technically and chemically?

Lösung anzeigen
TESTE DEIN WISSEN
  • technically it is produced by fermentation of ethanol with acetic acid bacteria (e.g. acetobacter) 
    • Surface process, Orleans process, fixed bed process or submersed process 
  • chemically it is produced under high pressure and temperature from methanol and carbon monoxide under catalytic influence of hydrogen iodide
Lösung ausblenden
TESTE DEIN WISSEN

Describe the production of one white biotechnology product

Lösung anzeigen
TESTE DEIN WISSEN
  • citric acid is produced in submerged fermentation and purified by removing the mycelia and calcium oxalate precipitation
  • citric acid is produced by A. niger
  • for that, the α-ketoglutarate dehydrogenase is downregulated by adding NH4+ and glucose
Lösung ausblenden
TESTE DEIN WISSEN

A non-glycosylated protein has a mass of 50.000 grams per mole; How many amino acids are in this protein and why?

Lösung anzeigen
TESTE DEIN WISSEN

- average weight of one amino acid: 110 g/mole

- 50.000/110 = 455 amino acids 

- the coding DNA is then 455*3 = 1365 base pairs

Lösung ausblenden
TESTE DEIN WISSEN

What is white biotechnology and what are typical products?

Lösung anzeigen
TESTE DEIN WISSEN

Industrial biotechnology 

e.g., acetic acid, butanol, acetone, citric acid, …

Lösung ausblenden
TESTE DEIN WISSEN

How can auxotroph mutants be isolated?

Lösung anzeigen
TESTE DEIN WISSEN
  • Cultivation on minimal media containing growth inhibiting antibiotics 
    • auxotroph mutants don´t grow on minimal media and are therefore not killed 
  • Planting on full medium 
  • Transfer to minimal media containing different supplements 
    • auxotroph mutants can grow with the missing supplement
Lösung ausblenden
  • 39005 Karteikarten
  • 1082 Studierende
  • 19 Lernmaterialien

Beispielhafte Karteikarten für deinen Selected Topics in Biotechnology Kurs an der Universität für Bodenkultur Wien - von Kommilitonen auf StudySmarter erstellt!

Q:

Biotechnology (colors)

A:
  • Green: agriculture, Plant BT 
  • White: Industry (industrial BT) 
  • Gray: waste management, environmental BT
  • Red: medical field, pharmaceutics, medical BT
  • Blue: exploitation of aquatic resources
Q:

What is red biotechnology and what are its typical products?

A:

medical biotechnology 

e.g., antibodies, insulin, …

Q:

What is subtilisin and how is it produced?

A:
  • Subtilisin are serin proteases from B. subtilis used as detergent enzymes
  • They are produced with a plasmid containing a gene for an antibiotic resistance
Q:

Describe the group of Biopolymers

A:

Polymers consisting of biologically produced molecules 

  • carbohydrates (starch, etc.…)
  • nucleic acids (chain of N-bases) 
  • proteins (amino acid chains)
Q:

How is butanol and acetone produced biotechnologically?

A:
  • Butanol and acetone can be produced by fermentation of anaerobic spore formers e.g. clostridia 
  • Corn is used as starting material and doesn´t need to be saccharified 
  • Fermentation runs strictly anaerobic under CO2 atmosphere
Mehr Karteikarten anzeigen
Q:

Describe the production of one red biotechnology product

A:
  • insulin can be produced recombinantly in S. cerevisiae 
  • the protein is secreted and can be purified with lysing the cells 
  • insulin is expressed with a shorter C-peptide which is easily cleaved off after purification
Q:

What substrates are suitable for ethanol production? What technical difficulties have to be considered?

A:

starch can be used as substrate, but it needs to be broken down by amylases and saccharified by glocoamylase

Q:

How is acetic acid produced ->technically and chemically?

A:
  • technically it is produced by fermentation of ethanol with acetic acid bacteria (e.g. acetobacter) 
    • Surface process, Orleans process, fixed bed process or submersed process 
  • chemically it is produced under high pressure and temperature from methanol and carbon monoxide under catalytic influence of hydrogen iodide
Q:

Describe the production of one white biotechnology product

A:
  • citric acid is produced in submerged fermentation and purified by removing the mycelia and calcium oxalate precipitation
  • citric acid is produced by A. niger
  • for that, the α-ketoglutarate dehydrogenase is downregulated by adding NH4+ and glucose
Q:

A non-glycosylated protein has a mass of 50.000 grams per mole; How many amino acids are in this protein and why?

A:

- average weight of one amino acid: 110 g/mole

- 50.000/110 = 455 amino acids 

- the coding DNA is then 455*3 = 1365 base pairs

Q:

What is white biotechnology and what are typical products?

A:

Industrial biotechnology 

e.g., acetic acid, butanol, acetone, citric acid, …

Q:

How can auxotroph mutants be isolated?

A:
  • Cultivation on minimal media containing growth inhibiting antibiotics 
    • auxotroph mutants don´t grow on minimal media and are therefore not killed 
  • Planting on full medium 
  • Transfer to minimal media containing different supplements 
    • auxotroph mutants can grow with the missing supplement
Selected Topics in Biotechnology

Erstelle und finde Lernmaterialien auf StudySmarter.

Greife kostenlos auf tausende geteilte Karteikarten, Zusammenfassungen, Altklausuren und mehr zu.

Jetzt loslegen

Das sind die beliebtesten StudySmarter Kurse für deinen Studiengang Selected Topics in Biotechnology an der Universität für Bodenkultur Wien

Für deinen Studiengang Selected Topics in Biotechnology an der Universität für Bodenkultur Wien gibt es bereits viele Kurse, die von deinen Kommilitonen auf StudySmarter erstellt wurden. Karteikarten, Zusammenfassungen, Altklausuren, Übungsaufgaben und mehr warten auf dich!

Das sind die beliebtesten Selected Topics in Biotechnology Kurse im gesamten StudySmarter Universum

Selected Topics in IT Security

Universität Mannheim

Zum Kurs
FOOD BIOTECHNOLOGY

Universiti Sains Malaysia

Zum Kurs
Biotechnology

Arab Academy for Science & Technology

Zum Kurs

Die all-in-one Lernapp für Studierende

Greife auf Millionen geteilter Lernmaterialien der StudySmarter Community zu
Kostenlos anmelden Selected Topics in Biotechnology
Erstelle Karteikarten und Zusammenfassungen mit den StudySmarter Tools
Kostenlos loslegen Selected Topics in Biotechnology