Molekularbiologie Übungen E.COLI at Universität für Bodenkultur Wien

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Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

You have a Plasmid with the following properties: an origin of

replication, an ampicillin resistance gene. You transform a small

amount of the Plasmid into E.coli and plate the Transformation mix on

nutrient media agar plates containing ampicillin. What will you see on

the plates after incubation overnight at 37°C?

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

In order to persist and be stably maintained in the cell, a Plasmid DNA

molecule must contain what?

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Which of the following is WRONG?

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Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Which of the following is true?

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Which of the following is WRONG?

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Which of the following is WRONG?

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Which of the following is WRONG?

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Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

GFP...

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

GFP....

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

GFP

Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Which of the following is true for DNA agarose gel electrophoresis?

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Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

A Plasmid is…

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Exemplary flashcards for Molekularbiologie Übungen E.COLI at the Universität für Bodenkultur Wien on StudySmarter:

Molekularbiologie Übungen E.COLI

You have a Plasmid with the following properties: an origin of

replication, an ampicillin resistance gene. You transform a small

amount of the Plasmid into E.coli and plate the Transformation mix on

nutrient media agar plates containing ampicillin. What will you see on

the plates after incubation overnight at 37°C?

colonies

Molekularbiologie Übungen E.COLI

In order to persist and be stably maintained in the cell, a Plasmid DNA

molecule must contain what?

an origin of replication

Molekularbiologie Übungen E.COLI

Which of the following is WRONG?

Alkaline Phosphatase from Shrimp is the most useful in molecular biology since it is the easiest to

inactivate after the reaction

Molekularbiologie Übungen E.COLI

Which of the following is true?

Bacteria can grow on medium containing ampicillin only when they have taken up a Plasmid that

carries a beta-lactamase gene.

Molekularbiologie Übungen E.COLI

Which of the following is WRONG?

Taq polymerase is one of many heat stable polymerases available on the market

Molekularbiologie Übungen E.COLI

Which of the following is WRONG?

In the T7 Expression System, the gene that encodes T7 polymerase is located on the genomic of the E.coli host cells. 

Molekularbiologie Übungen E.COLI

Which of the following is WRONG?

Electroporation is a widely used method for transformation

Molekularbiologie Übungen E.COLI

GFP...

was originally isolated from the green rainbow trout

Molekularbiologie Übungen E.COLI

GFP....

is the only fluorescent protein that is used in molecular biology research

Molekularbiologie Übungen E.COLI

GFP

is autofluorescent

Molekularbiologie Übungen E.COLI

Which of the following is true for DNA agarose gel electrophoresis?

Reducing the agarose concentration of a gel reduces the migration speed of the DNA fragments

Molekularbiologie Übungen E.COLI

A Plasmid is…

a double-stranded circular DNA molecule that is separate from the genomic DNA and that can

replicate independently

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