Introduction To Molecular Biology at Universität Für Bodenkultur Wien | Flashcards & Summaries

Lernmaterialien für Introduction to Molecular Biology an der Universität für Bodenkultur Wien

Greife auf kostenlose Karteikarten, Zusammenfassungen, Übungsaufgaben und Altklausuren für deinen Introduction to Molecular Biology Kurs an der Universität für Bodenkultur Wien zu.

TESTE DEIN WISSEN

Watson and Crick?

Lösung anzeigen
TESTE DEIN WISSEN
  • the composition of DNA varies from one species to another
  • total amounts of pyrimidine equal to the amounts of purine: A+G=C+T
  • after Frnklin and Gosline made a xray diffraction analysis they comined the information
    • Nucleotides from a linear chein, linked by phospodiester bonds
    • DNA froms a holical double strand
    • antiparallel organization
    • minor and major groove
    • 1 double helix turn - 10 bp
Lösung ausblenden
TESTE DEIN WISSEN

Identification of recombinant clones (electroporation)?

Lösung anzeigen
TESTE DEIN WISSEN
  • after electroporation
    • many Ecoli will have taken up one plasmid
    • very few will contain 2 oder more
    • many Ecoli cells without plasmid DNA are present
  • each plasmid molecule carries the selection marker gene (pET11a -> ampicillin)
  • on a medium containing ampicillin only Ecoli that carry a plasmid are able to grow
Lösung ausblenden
TESTE DEIN WISSEN

Histone Code?

Lösung anzeigen
TESTE DEIN WISSEN

numerous possible combinations

  • various effects on gene expression
  • highly dynamic and reversible
  • interaction between chromatin remodelling, modification histone variants -> impacts on gene expression


Lösung ausblenden
TESTE DEIN WISSEN

Gel Electrophoresis?

Lösung anzeigen
TESTE DEIN WISSEN
  • Gene materials:
    • argarose gel (routine application, longer than 200 bp)
    • polyacrylamide (short DNA fragments
  • DNA wanders to anode because of phosphate groups
  • sieve effect letzs long DNA fragments migrate more slowly
Lösung ausblenden
TESTE DEIN WISSEN

Regulation of DNA Replication?

Lösung anzeigen
TESTE DEIN WISSEN
  • Initiation of replication requires cis-acting elements: Origin of Replication (ORI)
  • preferentially A-T rich sequences - facilitated DNA helix opening
  • Initiator Proteins associate with ORI
  • promotes interaction with DNA Helicase
  • DNA Primase adds RNA primer
  • replication machinery starts with DNA replication in both directions
  • replication fork ist established
Lösung ausblenden
TESTE DEIN WISSEN

Replication in Eukaryotes?

Lösung anzeigen
TESTE DEIN WISSEN
  • typically longer and more complex genomes
  • organized as linear chromosomes
  • DNA replication and cell division is separated
  • DNA replication in S-phase, division in M-Phase
Lösung ausblenden
TESTE DEIN WISSEN

History of DNA?

Lösung anzeigen
TESTE DEIN WISSEN
  • Richard Dawkins first found out that genes represent fundamental units
  • DNA was first characterized by Friedrich Miescher who termed it Nucleic because it was found in the Nucleus
    • he actually thought that DNA respresents a structural compnent of chromosomes and didnt know its function
Lösung ausblenden
TESTE DEIN WISSEN

PCR Components?

Lösung anzeigen
TESTE DEIN WISSEN
  • Template
  • 2 Primers per template
  • Buffer (buffer substance, salt)
  • thermostable Polymerase (thermophillic bacteria - Thermus aquaticus, pyrococcus furiosus)
Lösung ausblenden
TESTE DEIN WISSEN

PCR History

Lösung anzeigen
TESTE DEIN WISSEN
  • first proposed by Khorana in 1970
  • made public by Kary Mullis in 1985
  • method of the year in 1989
  • nobel prize in 1993
Lösung ausblenden
TESTE DEIN WISSEN

Usage of primers of cDNA

Lösung anzeigen
TESTE DEIN WISSEN
  • Oligo dT
    • + primes on poly A+mRNA
    • + crude RNA preps
    • -Rere RNA can be underpresented
  • Random pdN6
    • +large cDNA library
    • - cDNA can be small in size
    • - poly A- template can also be reverse transcribed
  • Gene specific
    • + cDNa library is enriched for specific mRNA
    • cDNA libraried can be made for specific applications, cloning all antibody encoding
Lösung ausblenden
TESTE DEIN WISSEN

Visualizing the DNA in gel electrophoresis?

Lösung anzeigen
TESTE DEIN WISSEN

cluorescent dyes that bind to DNA and emit strong fluorescence upon excitation with UV light

  • classical dye: Ethidiumbromide
    • high sensivity
    • mutagenic and canverogenic
  • modern dyes: Sybrgreen, Sybrsafe, Midori Green
    • high sensitivity
    • safer -> less mutagenic
    • better for environment
Lösung ausblenden
TESTE DEIN WISSEN

DNA sequencing?

Lösung anzeigen
TESTE DEIN WISSEN
  • 2 types of DNA sequencing
    • Dideoxy cycle DNA sequencing:
      • for sequencing single clones
    • High troughput, next generation DNA sequencing:
      • for genome and transcriptome sequencing
Lösung ausblenden
  • 35111 Karteikarten
  • 1001 Studierende
  • 21 Lernmaterialien

Beispielhafte Karteikarten für deinen Introduction to Molecular Biology Kurs an der Universität für Bodenkultur Wien - von Kommilitonen auf StudySmarter erstellt!

Q:

Watson and Crick?

A:
  • the composition of DNA varies from one species to another
  • total amounts of pyrimidine equal to the amounts of purine: A+G=C+T
  • after Frnklin and Gosline made a xray diffraction analysis they comined the information
    • Nucleotides from a linear chein, linked by phospodiester bonds
    • DNA froms a holical double strand
    • antiparallel organization
    • minor and major groove
    • 1 double helix turn - 10 bp
Q:

Identification of recombinant clones (electroporation)?

A:
  • after electroporation
    • many Ecoli will have taken up one plasmid
    • very few will contain 2 oder more
    • many Ecoli cells without plasmid DNA are present
  • each plasmid molecule carries the selection marker gene (pET11a -> ampicillin)
  • on a medium containing ampicillin only Ecoli that carry a plasmid are able to grow
Q:

Histone Code?

A:

numerous possible combinations

  • various effects on gene expression
  • highly dynamic and reversible
  • interaction between chromatin remodelling, modification histone variants -> impacts on gene expression


Q:

Gel Electrophoresis?

A:
  • Gene materials:
    • argarose gel (routine application, longer than 200 bp)
    • polyacrylamide (short DNA fragments
  • DNA wanders to anode because of phosphate groups
  • sieve effect letzs long DNA fragments migrate more slowly
Q:

Regulation of DNA Replication?

A:
  • Initiation of replication requires cis-acting elements: Origin of Replication (ORI)
  • preferentially A-T rich sequences - facilitated DNA helix opening
  • Initiator Proteins associate with ORI
  • promotes interaction with DNA Helicase
  • DNA Primase adds RNA primer
  • replication machinery starts with DNA replication in both directions
  • replication fork ist established
Mehr Karteikarten anzeigen
Q:

Replication in Eukaryotes?

A:
  • typically longer and more complex genomes
  • organized as linear chromosomes
  • DNA replication and cell division is separated
  • DNA replication in S-phase, division in M-Phase
Q:

History of DNA?

A:
  • Richard Dawkins first found out that genes represent fundamental units
  • DNA was first characterized by Friedrich Miescher who termed it Nucleic because it was found in the Nucleus
    • he actually thought that DNA respresents a structural compnent of chromosomes and didnt know its function
Q:

PCR Components?

A:
  • Template
  • 2 Primers per template
  • Buffer (buffer substance, salt)
  • thermostable Polymerase (thermophillic bacteria - Thermus aquaticus, pyrococcus furiosus)
Q:

PCR History

A:
  • first proposed by Khorana in 1970
  • made public by Kary Mullis in 1985
  • method of the year in 1989
  • nobel prize in 1993
Q:

Usage of primers of cDNA

A:
  • Oligo dT
    • + primes on poly A+mRNA
    • + crude RNA preps
    • -Rere RNA can be underpresented
  • Random pdN6
    • +large cDNA library
    • - cDNA can be small in size
    • - poly A- template can also be reverse transcribed
  • Gene specific
    • + cDNa library is enriched for specific mRNA
    • cDNA libraried can be made for specific applications, cloning all antibody encoding
Q:

Visualizing the DNA in gel electrophoresis?

A:

cluorescent dyes that bind to DNA and emit strong fluorescence upon excitation with UV light

  • classical dye: Ethidiumbromide
    • high sensivity
    • mutagenic and canverogenic
  • modern dyes: Sybrgreen, Sybrsafe, Midori Green
    • high sensitivity
    • safer -> less mutagenic
    • better for environment
Q:

DNA sequencing?

A:
  • 2 types of DNA sequencing
    • Dideoxy cycle DNA sequencing:
      • for sequencing single clones
    • High troughput, next generation DNA sequencing:
      • for genome and transcriptome sequencing
Introduction to Molecular Biology

Erstelle und finde Lernmaterialien auf StudySmarter.

Greife kostenlos auf tausende geteilte Karteikarten, Zusammenfassungen, Altklausuren und mehr zu.

Jetzt loslegen

Das sind die beliebtesten StudySmarter Kurse für deinen Studiengang Introduction to Molecular Biology an der Universität für Bodenkultur Wien

Für deinen Studiengang Introduction to Molecular Biology an der Universität für Bodenkultur Wien gibt es bereits viele Kurse, die von deinen Kommilitonen auf StudySmarter erstellt wurden. Karteikarten, Zusammenfassungen, Altklausuren, Übungsaufgaben und mehr warten auf dich!

Das sind die beliebtesten Introduction to Molecular Biology Kurse im gesamten StudySmarter Universum

Molecular Biology

Universität für Bodenkultur Wien

Zum Kurs
Molecular Biology

Far Eastern University

Zum Kurs
Introductory cellular and molecular biology

Walter Sisulu University for Technology and Science

Zum Kurs
Molecular Biology

Fachhochschule Aachen

Zum Kurs
Introduction to Psychology

Open University of Sri Lanka

Zum Kurs

Die all-in-one Lernapp für Studierende

Greife auf Millionen geteilter Lernmaterialien der StudySmarter Community zu
Kostenlos anmelden Introduction to Molecular Biology
Erstelle Karteikarten und Zusammenfassungen mit den StudySmarter Tools
Kostenlos loslegen Introduction to Molecular Biology