Biological Therapeutics at Universität Für Bodenkultur Wien | Flashcards & Summaries

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Lernmaterialien für Biological Therapeutics an der Universität für Bodenkultur Wien

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Why can Insulin be produced in E coli?

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Because it only requires 3 Disulphide bonds, they can be established correctly in the DS process

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Where are glycosylated Proteins produced?

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E.Coli

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Where are 

  • IgG
  • IgA
  • IgE
  • IgM

found?

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  • IgG -> in body fluids
  • IgA -> protect from env., in vagina, nose, ears, eyes, digestive tract
  • IgE -> pollen, spores, protect from foreign substances, in lung and mucus membranes
  • IgM -> response to infection, in lymph and body fluids


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What makes the analysis of blood proteins so difficult?

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The extreme differences in concentration.

-> About 10^9

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Non mammalian production systems for antibodies still feature the drawback of not yielding the right glycosylation mixes. What can be one about that?

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Glycoenginering

  • Knockout of enzymes
  • Introduction of new enzymes
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The Fc region of a protein can br fuded to a protein of interest in order to increase its half-life. 

What protein would be an alternative to it?

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E.g the human albumin

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What is a side effect that must be expected with therapeutic antibodies?

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The acute infucion reaction.

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Some people might claim, that using human embryonic stem cell lines for the production of biological therapeutics would enable the production of completely human glycosylation (incl. the ability for bi-sectioned Glc-Nac). Why is this argument not ideal?

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It is not ideal, because the cell line will also have lost some (god knows which) abilities for glycosylation.
Alo, the use of human cells greatly increases the risk of a contamination with pathogens that can infect humans.

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What is the current maximum cell density, achievable with E.Coli? (in % V/V)

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40%

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How many glycosylation sites are there on IgM?

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5

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Which diseases are most often treated with mABs?

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  • Cancers
  • Immune diseases (rheumatoid arthritis)
    • or auto immune diseases in general
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What is the most abundant cell line used in pharma USP?

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CHO! (70%)

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Q:

Why can Insulin be produced in E coli?

A:

Because it only requires 3 Disulphide bonds, they can be established correctly in the DS process

Q:

Where are glycosylated Proteins produced?

A:

E.Coli

Q:

Where are 

  • IgG
  • IgA
  • IgE
  • IgM

found?

A:
  • IgG -> in body fluids
  • IgA -> protect from env., in vagina, nose, ears, eyes, digestive tract
  • IgE -> pollen, spores, protect from foreign substances, in lung and mucus membranes
  • IgM -> response to infection, in lymph and body fluids


Q:

What makes the analysis of blood proteins so difficult?

A:

The extreme differences in concentration.

-> About 10^9

Q:

Non mammalian production systems for antibodies still feature the drawback of not yielding the right glycosylation mixes. What can be one about that?

A:

Glycoenginering

  • Knockout of enzymes
  • Introduction of new enzymes
Mehr Karteikarten anzeigen
Q:

The Fc region of a protein can br fuded to a protein of interest in order to increase its half-life. 

What protein would be an alternative to it?

A:

E.g the human albumin

Q:

What is a side effect that must be expected with therapeutic antibodies?

A:

The acute infucion reaction.

Q:

Some people might claim, that using human embryonic stem cell lines for the production of biological therapeutics would enable the production of completely human glycosylation (incl. the ability for bi-sectioned Glc-Nac). Why is this argument not ideal?

A:

It is not ideal, because the cell line will also have lost some (god knows which) abilities for glycosylation.
Alo, the use of human cells greatly increases the risk of a contamination with pathogens that can infect humans.

Q:

What is the current maximum cell density, achievable with E.Coli? (in % V/V)

A:

40%

Q:

How many glycosylation sites are there on IgM?

A:

5

Q:

Which diseases are most often treated with mABs?

A:
  • Cancers
  • Immune diseases (rheumatoid arthritis)
    • or auto immune diseases in general
Q:

What is the most abundant cell line used in pharma USP?

A:

CHO! (70%)

Biological Therapeutics

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