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Why can Insulin be produced in E coli?
Because it only requires 3 Disulphide bonds, they can be established correctly in the DS process
Where are glycosylated Proteins produced?
E.Coli
Where are
found?
What makes the analysis of blood proteins so difficult?
The extreme differences in concentration.
-> About 10^9
Non mammalian production systems for antibodies still feature the drawback of not yielding the right glycosylation mixes. What can be one about that?
Glycoenginering
The Fc region of a protein can br fuded to a protein of interest in order to increase its half-life.
What protein would be an alternative to it?
E.g the human albumin
What is a side effect that must be expected with therapeutic antibodies?
The acute infucion reaction.
Some people might claim, that using human embryonic stem cell lines for the production of biological therapeutics would enable the production of completely human glycosylation (incl. the ability for bi-sectioned Glc-Nac). Why is this argument not ideal?
It is not ideal, because the cell line will also have lost some (god knows which) abilities for glycosylation.
Alo, the use of human cells greatly increases the risk of a contamination with pathogens that can infect humans.
What is the current maximum cell density, achievable with E.Coli? (in % V/V)
40%
How many glycosylation sites are there on IgM?
5
Which diseases are most often treated with mABs?
What is the most abundant cell line used in pharma USP?
CHO! (70%)
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