Tet1 at Universität Duisburg-Essen

Flashcards and summaries for Tet1 at the Universität Duisburg-Essen

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Study with flashcards and summaries for the course Tet1 at the Universität Duisburg-Essen

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

For which range of wavelenght measurements are quartz cuvettes useful?

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Name the formula used for the quantification of protein by UV/VIS spectroscopy in the presence of nucleic acids.

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?
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Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Name and explain the formula which can be used to calculate the energy of light.

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

"The ... the wavelenght of light, the higher its energy."

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

What is the working range of UV/VIS spectroscopy?

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Which wavelenghts give UV-C light?
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Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Which wavelenghts give UV-B light?

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Which wavelenghts give UV-A light?

Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Which wavelenghts give UV-A light?
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Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

How much energy does a molecule absorb from the UV/VIS light?

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Exemplary flashcards for Tet1 at the Universität Duisburg-Essen on StudySmarter:

Tet1

For which range of wavelenght measurements are quartz cuvettes useful?
> 200 nm

Tet1

Name the formula used for the quantification of protein by UV/VIS spectroscopy in the presence of nucleic acids.
Protein quantification by Warburg/Christian: Protein concentration = (1.55 * A280) - (0.76 * A260)

Tet1

On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?
The energy of UV/VIS light is large enough to promote the transition of valence electrons to higher energy levels. Hence, the molecule reaches an excited electronic state for a short time interval,

Tet1

Name and explain the formula which can be used to calculate the energy of light.
E = h * v = (h * c) / λ E = energy of a photon [J] h = 6.626 * 10(^-34) [Js] (Planck's constant) v = frequency [s^-1] λ = wavelenght [nm] c = 3 * 10^8 m/s (speed of light)

Tet1

"The ... the wavelenght of light, the higher its energy."
shorter

Tet1

What is the working range of UV/VIS spectroscopy?
200 - 800 nm

Tet1

Which wavelenghts give UV-C light?
200 - 280 nm

Tet1

Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?
Below 200 nm (mostly starting at 190 nm), the UV absorption of oxygen becomes significant and interferes with the measurement.

Tet1

Which wavelenghts give UV-B light?
280 - 320 nm

Tet1

Which wavelenghts give UV-A light?
320 - 400 nm

Tet1

Which wavelenghts give UV-A light?
320 - 400 nm

Tet1

How much energy does a molecule absorb from the UV/VIS light?
The energy of the absorbed light must equal the energy difference E between state 1 and 2.

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