Genetik at Universität Düsseldorf

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1. Mendelsche Regel

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Southern Blot 

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sex lethal und transformer

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enzymatische Sequenzierung (Sanger)

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Paarregelgene

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heterosomale Aneuploidien

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Expressivität
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Arbeitsschritt der Hybridisierung

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2 Klassen von P-Elementen 

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zellautonom 

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Aneuploidie
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Gap-Gene

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Exemplary flashcards for Genetik at the Universität Düsseldorf on StudySmarter:

Genetik

1. Mendelsche Regel
Uniformitätsgesetz
Kreuzt man reinerbige (homozygote) Individuen, die in einem Merkmal unterschiedlich sind (monohybrid), dann sind alle Nachkommen in der F1 Generation in diesem Merkmal gleich (uniform). 

Genetik

Southern Blot 
DNA 

Genetik

sex lethal und transformer

werden bei Drosophila anhand eines alternativen Spleißvorgangs in Weibchen aktiviert

Genetik

enzymatische Sequenzierung (Sanger)

Sequenzierung durch Synthetisierung 


Genetik

Paarregelgene

  • jedes 2. Segment betreffend (parasegmentale Einheiten)
  • werden von maternlen und Gap-Genen reguliert
  • aktivieren Expression der Segmentpolaritätsgene

Genetik

heterosomale Aneuploidien
  • Turner-Syndrom = Monosomie X 
  • Klinefelter-Syndrom = Trisomie XXY 
  • Trisomie XXX / XYY 

Genetik

Expressivität
Stärke der Ausprägung eines Phänotyps 

Genetik

Arbeitsschritt der Hybridisierung
  • Chromosome walking 
  • Southern blot
  • Chromosomen in-situ Hybridisierung 

Genetik

2 Klassen von P-Elementen 
P-Faktoren
mit funktionsfähiger Transposase
defekte P-Elemente 
ohne Transposasegen 
Verwendung bei Enhancer-trap 

Genetik

zellautonom 
signalempfangende Zelle 
der Phänotyp ist auf die eigentlichen mutanten Zellen beschränkt 

Genetik

Aneuploidie
Störung des normalen Diploidiegrads (Euploidie), Abweichung von der normalen Chromosomenzahl ganzer Chromosomensätze

Genetik

Gap-Gene

  • Verlust mehrerer benachbarter Segmenre
  • werden von Koordinatengenen reguliert
  • regulieren sich untereinander (jedes Gen reguliert mind. 2 andere)

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