Mol.971UF Microbiome in Health and Environment at Technische Universität Graz

Flashcards and summaries for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz

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Study with flashcards and summaries for the course Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Three examples of intramicrobiome microbial communication

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Modern Definition of Microbiome

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Viruses and Phages are Part of

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Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Name three interaction types of microbial interaction

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Three methods used for community fingerprinting

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Name Characteristics associated with Eubiosis and Dysbiosis

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

The cleaning regimen in a constricted environment like a hospital has what effect on the Microbiom?

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Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

5 Steps in the Workflow from Sample to (Meta)Genome or Amplicon.

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What markers are commonly used for Amplicon Sequencing?

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Name 4 Omic methods that complement each other in microbiome research

Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

What are the differences between Top Down, Middle down, Bottom up approaches in Proteomics

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Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Define core microbiota

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Exemplary flashcards for Mol.971UF Microbiome in Health and Environment at the Technische Universität Graz on StudySmarter:

Mol.971UF Microbiome in Health and Environment

Three examples of intramicrobiome microbial communication

QS - Quorum sensing (N-acyl-homoserine lactones or peptides) Phenotype adaptation/Biofilm formation

DIET Direct Interspecies Electron Transfer (DIET) important in anaerobic communties

VOCs - Volatile Organic Compounds: long term, long distance, cross kingdom

Mol.971UF Microbiome in Health and Environment

Modern Definition of Microbiome

Characteristic microbial community occupying a defined habitat with distinct physico-chemical properties. Consist of Microbiota + theater of activity and forms an ecological niche.

Mol.971UF Microbiome in Health and Environment

Viruses and Phages are Part of

Microbiome

Mol.971UF Microbiome in Health and Environment

Name three interaction types of microbial interaction

Positive (commensalism, mutualism, symbiotic)
Negative (amensalism, predation, parasitism, antagonism and competition)
Neutral.

Mol.971UF Microbiome in Health and Environment

Three methods used for community fingerprinting

DGGE/TGGE  Denaturing/Temperatur Gradient Gel Electrophoressis 

T-RFLP Terminal Restriction Fragments Length Polymorphism  

SSCP PCR-Single Strand Conformation Polymorphism

Mol.971UF Microbiome in Health and Environment

Name Characteristics associated with Eubiosis and Dysbiosis

Eubiosis: high diversity, high abundance, tight network, low presence of potential pathogens
Dysbiosis: low diversity, low abundance, loose network, high presence of potential pathogens

Mol.971UF Microbiome in Health and Environment

The cleaning regimen in a constricted environment like a hospital has what effect on the Microbiom?

Reduction of diversity, increase in abundance of antibiotic resistance. Shift from Gram+->Gram+.

Mol.971UF Microbiome in Health and Environment

5 Steps in the Workflow from Sample to (Meta)Genome or Amplicon.

1 Sampling (Sampling, Storing, Enrichment)
2 Processing (Extraction, Library prepartion)
3 Sequencing (Illumina, Pacbio, Oxford Nanopore)
4 Bioinformatics (Quality fitering, Clustering, Taxonomic/Functional Profiling, Comparative analysis, Visualisation
5 Publishing/Storing

Mol.971UF Microbiome in Health and Environment

What markers are commonly used for Amplicon Sequencing?

16S rRNA, 18S rRNA and ITS Internal trancribed spacers

Mol.971UF Microbiome in Health and Environment

Name 4 Omic methods that complement each other in microbiome research

Genomics (DNA)
Transcriptomics (RNA)
Proteomics (Proteins)
Metabolomics (Metabolites)

Mol.971UF Microbiome in Health and Environment

What are the differences between Top Down, Middle down, Bottom up approaches in Proteomics

Size of Protein/Peptide. Top-Down single Proteins are analyzed.
Middle down: Proteinmix is digested first, separated and peptides are analyzed
Bottom up: Proteinmix is digested into smaller fragments than middle down.

Mol.971UF Microbiome in Health and Environment

Define core microbiota

The stable species (and or functions) within a microbiome.

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Stress and Health

home environment (II)

Fundamentals of Environmental Health

human microbiome

environmental facts

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