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epithelial tissue can be present in 2 forms. what are they?
sheets of contiguous cells (epithelia) that cover the body on its external surface and line the body on its internal surface and (2) glands, which originate from invaginated epithelial cells.
location of simple columnar epithelium?
where are fascia adherens found?
Cardiac muscle cells
what allows for rapid exchange of nutrients and waste products carried by the extracellular fluid as it percolates through the ground substance?
water of hydration
What is fixation of a tissue?
refers to treatment of the tissue with chemical
agents that not only retard the alterations of the tissue
subsequent to death (or after removal from the body)
but also maintain its normal architecture.
What is the most common embedding medium?
paraffin. The tissue is placed in a suitable container of melted paraffin until it is completely infiltrated by that waxy substance.
Once the tissue is infiltrated, it is placed into a small
receptacle, covered with melted paraffin, and allowed
to harden, forming a paraffin block containing the tissue.
ECM is composed of
ground substance and fibers
Size of block used for mounting in light microscopy?
5 to 10 µm in thickness
What is light microscopy?
The light microscope is an instrument for visualizing fine detail of an object. It does this by creating a magnified image through the use of a series of glass lenses, which first focus a beam of light onto or through an object, and convex objective lenses to enlarge the image formed.
Components of protoplasm?
cytoplasm, extending from the plasma membrane to the nuclear envelope, and the karyoplasm, the material forming the contents of the nucleus.
How many classes of stains are there and what are they for?
3
Steps involved in preparing tissues for light microscopy?
Steps required in preparing tissues for light micros-
copy include (1) fixation, (2) dehydration and clearing,
(3) embedding, (4) sectioning, and (5) mounting and
staining the sections.
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