Micro Final at Louisiana State University | Flashcards & Summaries

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Lernmaterialien für Micro Final an der Louisiana State University

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Direct Microscopic Count

- Technique 

-  instrument used 

- how to count cell using this instrument

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- uses a specialized type of slide called cell counting cell or hemocytometer 

  • only the bacteria in certain portions of the chamber will be counted (cell number × dilution correction corresponding to area utilized
  • A direct count includes all cells, both live & dead

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Direct Microscopic Count

- Advantages & Disadvantages

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Advantages

Rapid (no incubation required) NO culturing therefore NO selection against certain organisms 

Disadvantages 

Counts live and dead cells 

Requires more skill

Requires more use of the hemocytometer 

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Colony forming units per millimeter (cfu/ml)
- equation
- definition 
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  • colony arises from one more than one cell, especially with cells that form clusters, pairs, or chain
cfu/ml (of original broth culture) = [# of colonies on cells] × [reciprocal of dilution factor]
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Countable Plate 
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  • one that yields 30-300 colonies
  • Plates with less than 30 colonies provide too few colonies to be significant or accurate
  • Plates with greater than 300 colonies are not considered valid because they may not have been considered adequate nutrients and space for all cells to grow to form colonies
Lösung ausblenden
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Turbidity
- increase in cells in broth how does this affect turbidty

- why? 

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  • microbial cells scatters light, broth cultures become cloudy as microorganisms grow
  • As number of cells in a broth increase, there is an increase in the turbidity of the solution
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Spectrophotometer

- definition 

- relationship between number of cells and amount of light  

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TESTE DEIN WISSEN
  • measures the amount of light (600 nm) that passes through a liquid
  • As number of cells in a broth increase, the amount of light in broth transmitted through the broth decreases
Lösung ausblenden
TESTE DEIN WISSEN
Turbidity Assay

- instrument 

- method used 

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TESTE DEIN WISSEN
uses Spectrophotometer (600 mn) and can create a standard curve from absorption values to find cfu/ ml
Lösung ausblenden
TESTE DEIN WISSEN
If Turbidity is correlated to viable cell counts and a standard curve is established so what happens 
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TESTE DEIN WISSEN
allows for a quick measurement can be used to determine the approximate number of cfu/ml
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TESTE DEIN WISSEN
What are the two types of Standard Plate Count? 

- advantages and disadvantages 

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TESTE DEIN WISSEN
Spread Plate: 
advantage: relatively simple to perform 
disadvantage: need good spread technique 
Pour Plate:
Advantages: No flames or alcohol involved
Disadvantaged: need melted tempered agar deeps; mostly evenly mix agar with culture
Lösung ausblenden
TESTE DEIN WISSEN
TurbidimetriC Assay

- advantages & disadvantages 

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TESTE DEIN WISSEN
Advantages: Rapid (no inculation required)
Disadvantages: Not accurate for microorganisms that grow in clumps 

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TESTE DEIN WISSEN

Yeast cells are rather large compared to most bacteria, making them easy to count all the cells in the entire grid of a cell counting chamber. What other organism you have viewed this semester could you easily count using a cell counting chamber? What characteristic of this organism might make it difficult to count?

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TESTE DEIN WISSEN

Chlamydomonas reinhardtii- large but they're mobile soooo you might have a hard time keeping them in place.

Lösung ausblenden
TESTE DEIN WISSEN

What does Enumerating mean and can it be achieved in several or simple steps?

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TESTE DEIN WISSEN

counting the number of cells in a sample can be used and achieved in several ways 

Lösung ausblenden
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Beispielhafte Karteikarten für deinen Micro Final Kurs an der Louisiana State University - von Kommilitonen auf StudySmarter erstellt!

Q:

Direct Microscopic Count

- Technique 

-  instrument used 

- how to count cell using this instrument

A:

- uses a specialized type of slide called cell counting cell or hemocytometer 

  • only the bacteria in certain portions of the chamber will be counted (cell number × dilution correction corresponding to area utilized
  • A direct count includes all cells, both live & dead

Q:

Direct Microscopic Count

- Advantages & Disadvantages

A:

Advantages

Rapid (no incubation required) NO culturing therefore NO selection against certain organisms 

Disadvantages 

Counts live and dead cells 

Requires more skill

Requires more use of the hemocytometer 

Q:
Colony forming units per millimeter (cfu/ml)
- equation
- definition 
A:
  • colony arises from one more than one cell, especially with cells that form clusters, pairs, or chain
cfu/ml (of original broth culture) = [# of colonies on cells] × [reciprocal of dilution factor]
Q:
Countable Plate 
A:
  • one that yields 30-300 colonies
  • Plates with less than 30 colonies provide too few colonies to be significant or accurate
  • Plates with greater than 300 colonies are not considered valid because they may not have been considered adequate nutrients and space for all cells to grow to form colonies
Q:
Turbidity
- increase in cells in broth how does this affect turbidty

- why? 

A:
  • microbial cells scatters light, broth cultures become cloudy as microorganisms grow
  • As number of cells in a broth increase, there is an increase in the turbidity of the solution
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Q:
Spectrophotometer

- definition 

- relationship between number of cells and amount of light  

A:
  • measures the amount of light (600 nm) that passes through a liquid
  • As number of cells in a broth increase, the amount of light in broth transmitted through the broth decreases
Q:
Turbidity Assay

- instrument 

- method used 

A:
uses Spectrophotometer (600 mn) and can create a standard curve from absorption values to find cfu/ ml
Q:
If Turbidity is correlated to viable cell counts and a standard curve is established so what happens 
A:
allows for a quick measurement can be used to determine the approximate number of cfu/ml
Q:
What are the two types of Standard Plate Count? 

- advantages and disadvantages 

A:
Spread Plate: 
advantage: relatively simple to perform 
disadvantage: need good spread technique 
Pour Plate:
Advantages: No flames or alcohol involved
Disadvantaged: need melted tempered agar deeps; mostly evenly mix agar with culture
Q:
TurbidimetriC Assay

- advantages & disadvantages 

A:
Advantages: Rapid (no inculation required)
Disadvantages: Not accurate for microorganisms that grow in clumps 

Q:

Yeast cells are rather large compared to most bacteria, making them easy to count all the cells in the entire grid of a cell counting chamber. What other organism you have viewed this semester could you easily count using a cell counting chamber? What characteristic of this organism might make it difficult to count?

A:

Chlamydomonas reinhardtii- large but they're mobile soooo you might have a hard time keeping them in place.

Q:

What does Enumerating mean and can it be achieved in several or simple steps?

A:

counting the number of cells in a sample can be used and achieved in several ways 

Micro Final

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