Clinical Applications Of Biomedical Sciences at Keele University | Flashcards & Summaries

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What is biomedical science WEEK 2
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Biomedical science is divided intro four different laboratory disciplines: 

1. Infection sciences: microbiology and virology 

2. Blood sciences: clinical chemistry , haematology,  transfusion and immunology 

3. Cell sciences: histology and cytology 

4. Genetics and molecular pathology 
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Medical qualified pathologists WEEK 2 
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Initial cut up, interpretation is f slides , dictating macroscopic and microscopic findings. 
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What Secretarial staff do WEEK 2
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Entering of patients onto the LIMS,

transcribing of macroscopic descriptions 
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What is the first step following sample collection?
WEEK 3


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Is fixation 
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Osmotic concentration WEEK 3
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Often salts are added to fixatives to produce isotonic solutions that do not result in cell shrinkage or swelling 
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Fixation and decalcifixation WEEK 3
What is autolysis and putrefaction:


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TESTE DEIN WISSEN
Autolysis: the breakdown of plant or animal tissue by action of enzymes contained within the affected tissue(autologous). 


Putrefaction: Decomposition produced, in the most part, by the action of bacterial enzymes derived from the bowel. Enzymes from fungi may also contribute to this process 
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Roles and responsibilities.  WEEK 2
What is a laboratory assistant 
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Booking in of specimens, ordering of supplies 
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Biomedical scientists: WEEK 2
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Processing, embedding, sectioning and staying specimens and have control of control and quality assurance. 
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Why do we stain tissues WEEK 4
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In order to observe in detail the tissues and their different features 
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What does fixation do. WEEK 3
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TESTE DEIN WISSEN
Fixation aims to maintain cells in as life like stage as possible following their removal from the body. It is concerned with stabilizing the proteins within a tissue and preventing the effects of putrefaction. 
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TESTE DEIN WISSEN
Examples of   WEEK 3

Cross linking additive fixatives: 


Precipitating non additive fixatives: 
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TESTE DEIN WISSEN
Cross linking additive fixatives:
 
formaldehyde 
Gluteraldehyde 
Osmium tetroxide 

Precipitating non additive fixatives:

Alcohol 
Methanol 
Acetone 
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TESTE DEIN WISSEN
mechanisms of fixation: WEEK 3

Cross-linking (additive)fixatives: 

Precipitating (non-additive)fixatives 
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TESTE DEIN WISSEN
Cross-linking (additive)fixatives:
These bind amino acids in proteins to adjust their tertiary structure thus preventing autolysis and putrefaction. 


Precipitating (non-additive)fixatives:
These act by removing water from the cellular matrix, thus disrupting the tertiary structure of proteins resulting in precipitation. 
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Q:
What is biomedical science WEEK 2
A:
Biomedical science is divided intro four different laboratory disciplines: 

1. Infection sciences: microbiology and virology 

2. Blood sciences: clinical chemistry , haematology,  transfusion and immunology 

3. Cell sciences: histology and cytology 

4. Genetics and molecular pathology 
Q:
Medical qualified pathologists WEEK 2 
A:
Initial cut up, interpretation is f slides , dictating macroscopic and microscopic findings. 
Q:
What Secretarial staff do WEEK 2
A:
Entering of patients onto the LIMS,

transcribing of macroscopic descriptions 
Q:
What is the first step following sample collection?
WEEK 3


A:
Is fixation 
Q:
Osmotic concentration WEEK 3
A:
Often salts are added to fixatives to produce isotonic solutions that do not result in cell shrinkage or swelling 
Mehr Karteikarten anzeigen
Q:
Fixation and decalcifixation WEEK 3
What is autolysis and putrefaction:


A:
Autolysis: the breakdown of plant or animal tissue by action of enzymes contained within the affected tissue(autologous). 


Putrefaction: Decomposition produced, in the most part, by the action of bacterial enzymes derived from the bowel. Enzymes from fungi may also contribute to this process 
Q:
Roles and responsibilities.  WEEK 2
What is a laboratory assistant 
A:
Booking in of specimens, ordering of supplies 
Q:
Biomedical scientists: WEEK 2
A:
Processing, embedding, sectioning and staying specimens and have control of control and quality assurance. 
Q:
Why do we stain tissues WEEK 4
A:
In order to observe in detail the tissues and their different features 
Q:
What does fixation do. WEEK 3
A:
Fixation aims to maintain cells in as life like stage as possible following their removal from the body. It is concerned with stabilizing the proteins within a tissue and preventing the effects of putrefaction. 
Q:
Examples of   WEEK 3

Cross linking additive fixatives: 


Precipitating non additive fixatives: 
A:
Cross linking additive fixatives:
 
formaldehyde 
Gluteraldehyde 
Osmium tetroxide 

Precipitating non additive fixatives:

Alcohol 
Methanol 
Acetone 
Q:
mechanisms of fixation: WEEK 3

Cross-linking (additive)fixatives: 

Precipitating (non-additive)fixatives 
A:
Cross-linking (additive)fixatives:
These bind amino acids in proteins to adjust their tertiary structure thus preventing autolysis and putrefaction. 


Precipitating (non-additive)fixatives:
These act by removing water from the cellular matrix, thus disrupting the tertiary structure of proteins resulting in precipitation. 
Clinical Applications of Biomedical Sciences

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