Biotechnology at Hochschule Rhein-Waal

Flashcards and summaries for Biotechnology at the Hochschule Rhein-Waal

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Hybridization 

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Extension/Elongation

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What are molecular scissors and what are they obtained from?
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Exemplary flashcards for Biotechnology at the Hochschule Rhein-Waal on StudySmarter:

What is exonucleases?

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What is endonucleases?

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What is biotechnology?

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What are cloning vectors?
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Exemplary flashcards for Biotechnology at the Hochschule Rhein-Waal on StudySmarter:

Examples of cloning vectors

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What is recombinant DNA?

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What does PCR stand for?

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How is PCR useful in biotechnology?
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The phenomenon that provides opportunities for variations and formulation of unique combinations of genetic set up  is

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Exemplary flashcards for Biotechnology at the Hochschule Rhein-Waal on StudySmarter:

Biotechnology

Hybridization 
Primers stick to DNA strands 

Biotechnology

Extension/Elongation
DNA copying enzyme becomes active. It grabs onto annealed primer and extends strand using free-floating dNTPs, thus synthesizing complementary strand of DNA

Biotechnology

What are molecular scissors and what are they obtained from?
Restriction endonucleases are called molecular scissors and they are obtained from bacterial cells

Biotechnology

What is exonucleases?
. These break the dna from the ends
. They remove nucleotides from the ends of the dna
.the separated fragments are not used in genetic engineering process.

Biotechnology

What is endonucleases?
. These cut dna from inside
. They remove nucleotides from specific positions of within dna
. The desirable fragments are used in genetic engineering method.

Biotechnology

What is biotechnology?
The branch of science that deals with utilization of properties and uses of micro organisms
Exploitation of their cells and cell constituents at the industrial level to generate essential products for human welfare

Biotechnology

What are cloning vectors?
The self replicative dna used to transfer alien dna into a suitable host cell for cloning is called cloning vector

Biotechnology

Examples of cloning vectors
Bacteriophages,plasmids and cosmids 

Biotechnology

What is recombinant DNA?
The DNA into which the gene of interest is lighted is called recombinant DNA. 
The construction of recombinant DNA  requires  the enzyme DNA  lipase. 

Biotechnology

What does PCR stand for?
Polymerised chain reaction 

Biotechnology

How is PCR useful in biotechnology?
PCR technique is used in 
. DNA  cloning
. Gene amplification 
. Diagnosis of hereditary diseases
. DNA finger printing process

Biotechnology

The phenomenon that provides opportunities for variations and formulation of unique combinations of genetic set up  is
Sexual reproduction 

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