Biotechnology at Hochschule Rhein-Waal | Flashcards & Summaries

Biotechnology at Hochschule Rhein-Waal

Flashcards and summaries for Biotechnology at the Hochschule Rhein-Waal

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Leading strand 

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Allele 

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What is exonucleases?
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To make host bacterial competent (the take up r DNA) the cells are treated with 

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What is biotechnology?

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Chromosomes

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The enzymes used in the construction  of r DNA  are 
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Sexual reproduction  permits 

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Extension/Elongation

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The method used in the amplification of gene of interest is

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via fermentation, anaerobic organisms can...
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Affinity chromatography 

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Exemplary flashcards for Biotechnology at the Hochschule Rhein-Waal on StudySmarter:

Biotechnology

Leading strand 
Polymerase follows helicase and synthesizes one continuous strand of dna

Biotechnology

Allele 
Any of several forms of a gene, usually arising through mutation, that are responsible for hereditary variation

some genes have more than two alleles 

Biotechnology

What is exonucleases?
. These break the dna from the ends
. They remove nucleotides from the ends of the dna
.the separated fragments are not used in genetic engineering process.

Biotechnology

To make host bacterial competent (the take up r DNA) the cells are treated with 
Dilute solution of calcium chloride solution ( calcium,a divalent cation )

Biotechnology

What is biotechnology?
The branch of science that deals with utilization of properties and uses of micro organisms
Exploitation of their cells and cell constituents at the industrial level to generate essential products for human welfare

Biotechnology

Chromosomes
  • Supercoiled dna
  • Millions of basepairs
  • 23 chromosomes, whole genetic material in human body, present in every cell

Biotechnology

The enzymes used in the construction  of r DNA  are 
Restriction enzyme and DNA ligase

Biotechnology

Sexual reproduction  permits 
Variations

Biotechnology

Extension/Elongation
DNA copying enzyme becomes active. It grabs onto annealed primer and extends strand using free-floating dNTPs, thus synthesizing complementary strand of DNA

Biotechnology

The method used in the amplification of gene of interest is
PCR method

Biotechnology

via fermentation, anaerobic organisms can...
produce acids which will lower the pH

Biotechnology

Affinity chromatography 
A method, that can be used to purify & separate crude mixture of proteins based on their affinities to certain molecules

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