Instrumentelle Analytik at Hochschule Hamm-Lippstadt

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Exemplary flashcards for Instrumentelle Analytik at the Hochschule Hamm-Lippstadt on StudySmarter:

How can you increase the accuracy of a DAD?

Exemplary flashcards for Instrumentelle Analytik at the Hochschule Hamm-Lippstadt on StudySmarter:

Def: Spezifität

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Which valence electrons are considered to be n-electrons?
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Exemplary flashcards for Instrumentelle Analytik at the Hochschule Hamm-Lippstadt on StudySmarter:

In UV/VIS measurements, which light source should be used for expected wavelenghts of 290-900 nm?

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Put the following side chains in order according to extinction coefficient ∊: Phenylalanine, Tyrosine, Tryptophan

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What is the major application of UV/VIS?

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For which range of wavelenght measurements are plastic cuvettes useful?
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Exemplary flashcards for Instrumentelle Analytik at the Hochschule Hamm-Lippstadt on StudySmarter:

Why are the absorption bands broad?

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Which wavelenghts give UV-A light?

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Which wavelenghts give VIS light?

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How do you interpret an absorbance value of A > 1.0?
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Which wavelenghts give UV-C light?

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Exemplary flashcards for Instrumentelle Analytik at the Hochschule Hamm-Lippstadt on StudySmarter:

Instrumentelle Analytik

How can you increase the accuracy of a DAD?
By increasing the number of diodes

Instrumentelle Analytik

Def: Spezifität

Die Spezifität gibt an wie von möglichen Störfaktoren die Probe ist.
Denn eine Methode ist nur 100% spezifischen wenn die Methode vollkommen frei von Störfaktoren in der Probe ist (ist theoretische Größe, da es immer Störfaktoren gibt)

Instrumentelle Analytik

Which valence electrons are considered to be n-electrons?
non bonding, free electron pairs

Instrumentelle Analytik

In UV/VIS measurements, which light source should be used for expected wavelenghts of 290-900 nm?
To show this wavelenght range (VIS-range), a Tungsten-halogen lamp should be used.

Instrumentelle Analytik

Put the following side chains in order according to extinction coefficient ∊: Phenylalanine, Tyrosine, Tryptophan
Tryptophan (∊ = 6000 l/mol cm) > Tyrosine (∊ = 1500 l/mol cm) > Phenylalanine (∊ = 200 l/mol cm)

Instrumentelle Analytik

What is the major application of UV/VIS?
Quantification of analytes e.g. proteins or DNA, also enzyme kinetics

Instrumentelle Analytik

For which range of wavelenght measurements are plastic cuvettes useful?
> 400 nm

Instrumentelle Analytik

Why are the absorption bands broad?
It is possible to excite vibrations and rotations of the molecule along with the excitation of electrons (vibrational levels between electronic ground states). This leads to small variations in the energy absorbed.

Instrumentelle Analytik

Which wavelenghts give UV-A light?
320 - 400 nm

Instrumentelle Analytik

Which wavelenghts give VIS light?
400 - 800 nm

Instrumentelle Analytik

How do you interpret an absorbance value of A > 1.0?
If A > 1.0, less than 10% of light intensity reaches the detector, which impairs the accuracy of quantitative measurement.

Instrumentelle Analytik

Which wavelenghts give UV-C light?
200 - 280 nm

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