Instrumental Analysis at Hochschule Bonn-Rhein-Sieg

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Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

"The ... the wavelenght of light, the higher its energy."

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?
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Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which wavelenghts give UV-A light?

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Which wavelenghts give VIS light?

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Which colours can molecules have for absorptions below 400 nm?

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What colour do coloured compounds show after absorption of VIS light?
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What are the three types of valence electrons?

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Which valence electrons are considered σ-electrons?

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Which valence electrons are considered to be π-electrons?

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Which valence electrons are considered to be n-electrons?
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Why are n to σ* rarely seen in UV/VIS spectroscopy?

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Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Instrumental Analysis

On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?
The energy of UV/VIS light is large enough to promote the transition of valence electrons to higher energy levels. Hence, the molecule reaches an excited electronic state for a short time interval,

Instrumental Analysis

"The ... the wavelenght of light, the higher its energy."
shorter

Instrumental Analysis

Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?
Below 200 nm (mostly starting at 190 nm), the UV absorption of oxygen becomes significant and interferes with the measurement.

Instrumental Analysis

Which wavelenghts give UV-A light?
320 - 400 nm

Instrumental Analysis

Which wavelenghts give VIS light?
400 - 800 nm

Instrumental Analysis

Which colours can molecules have for absorptions below 400 nm?
Molecules which only absorb UV light are colourless. Only molecules which absorb VIS light show colour.

Instrumental Analysis

What colour do coloured compounds show after absorption of VIS light?
They appear in the complementary colour. If the light of two complementary colours is mixed, white results, if one is taken away, the other is shown. Example: A substance is irritiated with white light. This compound absorbs red light at 700 nm. All other colours of white light are reflected. The reflected colour is green because the complementary colour red is missing.

Instrumental Analysis

What are the three types of valence electrons?
σ-electrons, π-electrons and n-electrons

Instrumental Analysis

Which valence electrons are considered σ-electrons?
single bonds

Instrumental Analysis

Which valence electrons are considered to be π-electrons?
double and triple bonds

Instrumental Analysis

Which valence electrons are considered to be n-electrons?
non bonding, free electron pairs

Instrumental Analysis

Why are n to σ* rarely seen in UV/VIS spectroscopy?
Compounds containing unshared electron pairs (e.g. O, S, N or halogens such as alcohols, amines, thiols) typically absorb in the range of 150-250 nm, but not very common above 200 nm, hence not in the UV range.

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