Instrumental Analysis at Hochschule Bonn-Rhein-Sieg

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Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How much energy does a molecule absorb from the UV/VIS light?

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name and explain the formula which can be used to calculate the energy of light.

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

"The ... the wavelenght of light, the higher its energy."

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is the working range of UV/VIS spectroscopy?

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?

Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which wavelenghts give UV-C light?

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Which wavelenghts give UV-B light?

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Which wavelenghts give UV-A light?

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Which wavelenghts give UV-A light?

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Which wavelenghts give VIS light?

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Which colours can molecules have for absorptions below 400 nm?

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Exemplary flashcards for Instrumental Analysis at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Instrumental Analysis

On a molecular level, what happens to a molecule that absorbs ultraviolet or visible light?
The energy of UV/VIS light is large enough to promote the transition of valence electrons to higher energy levels. Hence, the molecule reaches an excited electronic state for a short time interval,

Instrumental Analysis

How much energy does a molecule absorb from the UV/VIS light?
The energy of the absorbed light must equal the energy difference E between state 1 and 2.

Instrumental Analysis

Name and explain the formula which can be used to calculate the energy of light.
E = h * v = (h * c) / λ E = energy of a photon [J] h = 6.626 * 10(^-34) [Js] (Planck's constant) v = frequency [s^-1] λ = wavelenght [nm] c = 3 * 10^8 m/s (speed of light)

Instrumental Analysis

"The ... the wavelenght of light, the higher its energy."
shorter

Instrumental Analysis

What is the working range of UV/VIS spectroscopy?
200 - 800 nm

Instrumental Analysis

Why does UV/VIS spectroscopy not work for wavelenghts below 200 nm?
Below 200 nm (mostly starting at 190 nm), the UV absorption of oxygen becomes significant and interferes with the measurement.

Instrumental Analysis

Which wavelenghts give UV-C light?
200 - 280 nm

Instrumental Analysis

Which wavelenghts give UV-B light?
280 - 320 nm

Instrumental Analysis

Which wavelenghts give UV-A light?
320 - 400 nm

Instrumental Analysis

Which wavelenghts give UV-A light?
320 - 400 nm

Instrumental Analysis

Which wavelenghts give VIS light?
400 - 800 nm

Instrumental Analysis

Which colours can molecules have for absorptions below 400 nm?
Molecules which only absorb UV light are colourless. Only molecules which absorb VIS light show colour.

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