Immunology (Practical Course) at Hochschule Bonn-Rhein-Sieg

Flashcards and summaries for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg

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Study with flashcards and summaries for the course Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What does MACS stand for?

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is MACS used for?

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name the three components of the MACS.

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which column will be used for the MACS during practical course?

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

"The principle of MACS is a highly specific method, but also very time consuming" - Right or wrong?

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How many target cells can be sorted up using an MS column during MACS out of a total cell number of 2 * 10^8?

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name the three steps of the MACS procedure.

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name and explain the first step of the MACS procedure.

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name and explain the second step of the MACS procedure.

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name and explain the third step of the MACS procedure.

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name the three different cell separation strategies offered by MACS.

Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How do you perform a positive selection using MACS?

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Exemplary flashcards for Immunology (Practical Course) at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Immunology (Practical Course)

What does MACS stand for?
Magnetic activated cell sorting

Immunology (Practical Course)

What is MACS used for?
Magnetic activated cell sorting (MACS) is a gentle and highly specific method to isolate cell types of interest.

Immunology (Practical Course)

Name the three components of the MACS.
1.) Microbeads → paramagnetic particles coupled to e.g. antibody, about 50 nm in diameter, composed of biodegradable matrix 2.) Separator → strong permanent magnet 3.) Column → composed of ferromagnetic spheres, strongly amplifies the magnetic field when placed in separator, different column capacities available

Immunology (Practical Course)

Which column will be used for the MACS during practical course?
MS column

Immunology (Practical Course)

"The principle of MACS is a highly specific method, but also very time consuming" - Right or wrong?
Wrong. It is true that MACS is highly specific, but is also very simple and fast in performance.

Immunology (Practical Course)

How many target cells can be sorted up using an MS column during MACS out of a total cell number of 2 * 10^8?
1 * 10^7 target cells can be sorted out of 2 * 10^8 cells in total, yielding a purity of up to 99%.

Immunology (Practical Course)

Name the three steps of the MACS procedure.
1.) Magnetic labelling 2.) Magnetic separation 3.) Elution of retained cell population

Immunology (Practical Course)

Name and explain the first step of the MACS procedure.
Step 1: Magnetic labelling → The cell type of interest is magnetically labelled with an antibody (MicroBead complex).

Immunology (Practical Course)

Name and explain the second step of the MACS procedure.
Step 2: Magnetic separation → The column is placed in the separator. Upon loading, magnetically labeled cells are retained in the column while unlabeled cells are washed away and collected in the flow through.

Immunology (Practical Course)

Name and explain the third step of the MACS procedure.
Step 3: Elution of retained cell population → The column is removed from the separator. The cells of interest are eluted from the column as a highly enriched and pure fraction.

Immunology (Practical Course)

Name the three different cell separation strategies offered by MACS.
1.) Positive selection 2.) Depletion 3.) Untouched isolation

Immunology (Practical Course)

How do you perform a positive selection using MACS?
→ Cell type of interest is magnetically labeled → Not retained in column but elution → Cells of interest present in elution of flow through

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