IA at Hochschule Bonn-Rhein-Sieg

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Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is the working range of UV/VIS?

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What happens at a wavelength below 190 nm?

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is the color of molecules which absorb in the UV range?

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What happens if the light of two complementary colors is mixed?

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which types of valence electrons do organic molecules contain?

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which transition requires the least amount of energy and is therefore usually most important?

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

sigma to sigma* transition

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

n to sigma*

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Which substance can be used as solvent for UV/VIS and why?

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Pi to Pi*

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Chromophores

Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

n to pi*

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Exemplary flashcards for IA at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

IA

What is the working range of UV/VIS?
200-800 nm

IA

What happens at a wavelength below 190 nm?
The UV absorption of oxygen becomes significant and interferes with the measurement (vacuum-UV range)

IA

What is the color of molecules which absorb in the UV range?
colorless

IA

What happens if the light of two complementary colors is mixed?
White light results

IA

Which types of valence electrons do organic molecules contain?
- sigma electrons (single bonds) - pi electrons (double and triple bonds) - n-electrons (non bonding, free electron pairs)

IA

Which transition requires the least amount of energy and is therefore usually most important?
The transition from the highen occupied molecular orbital (HOMO) to the lowest unoccupied molecular orbital (LUMO)

IA

sigma to sigma* transition
- Highest ΔE values --> lowest wavelength - absorbance maxima: below 150 nm (in vacuum-UV range) - Ex.: C-C bond, C-H bonds (alkanes)

IA

n to sigma*
- Absorbe in the range of 150-250 nm (not very common above 200 nm) - Compound must contain atoms with unshared electron pairs, e.g. O, S, N or halogen, such as alcohols, amines, thiols

IA

Which substance can be used as solvent for UV/VIS and why?
- Water and alcohol, because they do not show absorption bands above 200 nm (used for polar analytes) - hexane or cyclohexane (for unpolar analytes)

IA

Pi to Pi*
- many functional groups absorb in the range of 200-700 nm - Typical structural unit: Carbonyl-groups (C=O), CC double bonds (esp. conjugated systems)

IA

Chromophores
Structural unit of a molecule which is responsible for the absorption of light in the UV/VIS range - e.g. conjugated pi-bonds - chromophores themselves or in combination with autochromes (e.g. amino group) can absorb light in the visible range, so that the compound appears colored

IA

n to pi*
- compound must contain atoms with unshared electron pairs (e.g. O, S, N or halogen) and additionally pi-electrons (e.g. double bond) - Typical structural unit: Carbonyl groups (C=O) --> usually produce two different absorption bands in the spectrum due to pi to pi* and n to pi* transition

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