human biology/ histology at Hochschule Bonn-Rhein-Sieg

Flashcards and summaries for human biology/ histology at the Hochschule Bonn-Rhein-Sieg

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Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

histology?(from Greek )

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

what are the Aims? 

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Tissue preparation for histology

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

1. Fixation

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Fixation Physical methods:

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Fixation Chemical methods:

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Coagulant fixatives

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Non-coagulant fixatives ....

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Commonly used chemical fixatives: coagulant 

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Commonly used chemical fixatives: non-coagulant

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Coagulant fixatives produce ...

Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Sectioning: problem

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Exemplary flashcards for human biology/ histology at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

human biology/ histology

histology?(from Greek )

histos = tissue („Gewebe“) and logos = knowledge

human biology/ histology

what are the Aims? 

Identifying and describing in detail the basic types of tissues.


 Knowledge of the most important organs and organ systems of the human body and describing their macroscopic and microscopic structure. 


Understanding of structure-function relationships of tissues and organs

human biology/ histology

Tissue preparation for histology

1. Fixation 2. Sectioning  3. Staining

human biology/ histology

1. Fixation

  • Prevention of autolysis
  • Termination of cell metabolism
  • Prevention of putrefaction (Putrefaction: degradation by microorganisms (bacteria, funghi) 


Fixation is mainly based on the denaturation and/or crosslinking of intracellular proteins  

human biology/ histology

Fixation Physical methods:

  • Drying 
  • Freezing 
  • Heating (Microwave)

human biology/ histology

Fixation Chemical methods:

  • coagulant fixatives 
  • non-coagulant fixatives

human biology/ histology

Coagulant fixatives

Coagulant fixatives produce a permeable meshwork of proteins

human biology/ histology

Non-coagulant fixatives ....

.....produce a less permeable gel

human biology/ histology

Commonly used chemical fixatives: coagulant 

Picric acid

human biology/ histology

Commonly used chemical fixatives: non-coagulant


Glutaraldehyde

human biology/ histology

Coagulant fixatives produce ...

produce a permeable meshwork of proteins

human biology/ histology

Sectioning: problem

Problem: fresh tissue is usually to soft to allow preparation of thin sections

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