Cell Culture at Hochschule Bonn-Rhein-Sieg

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Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are adherent cells?

Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What happens if adherent cell cultures become confluent?

Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is special about suspension cells?

Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which cells can be grown in suspensions?

Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Who did the first cell culture, when and with which organism?

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Which cell line is very popular and lives until this day?

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Mention the main events of the HeLa story!

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What is a organ culture?

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What is an explant culture?

Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What is a dissociated culture?

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What is an organotypic culture?

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Which cell lines can be split only a finite number of times before senescence and death?

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Exemplary flashcards for Cell Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Cell Culture

What are adherent cells?
Cells that are anchorage-dependent and propagate as a monolayer attached to the culture vessel

Cell Culture

What happens if adherent cell cultures become confluent?
They will cease proliferation and some will die when they in this state for too long

Cell Culture

What is special about suspension cells?
They can survive and proliferate without being attached to a substratum

Cell Culture

Which cells can be grown in suspensions?
Hematopoietic cells (derived from blood, spleen or bone marrow) as well as some transformed cell lines derived from malignant tumors

Cell Culture

Who did the first cell culture, when and with which organism?
1907: Harrison, frosh tissue (approx. 50 years later)

Cell Culture

Which cell line is very popular and lives until this day?
HeLa (1952)

Cell Culture

Mention the main events of the HeLa story!
- 1951: the first immortal human cell line was created - 20000 tubes of HeLa cells were supplied - 1970: HeLa cells had contaminated most established cell lines (USA) - since 2001: 5 novel prices for HeLa cells - 11000 patents - 50 Mio tons of tissue created

Cell Culture

What is a organ culture?
Tissue at gas-liquid interphase, histological structure maintained

Cell Culture

What is an explant culture?
Tissue at solid interphase; cells migrate to form outgrowth

Cell Culture

What is a dissociated culture?
Disaggregated tissue; cells form monolayer at solid-liquid interphase

Cell Culture

What is an organotypic culture?
Different cells cocultured with or without matrix; organotypic structure recreated

Cell Culture

Which cell lines can be split only a finite number of times before senescence and death?
Normal cell lines

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