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Cell Culture - Lecture 8: Gene Transfer
What are the two types of transfection?
Cell Culture - Lecture 8: Gene Transfer
For which type of transfection are plasmids used?
Transient
Cell Culture - Lecture 8: Gene Transfer
What are barriers within the cell that may prevent transfer of exogenous DNA?
Cell Culture - Lecture 8: Gene Transfer
By which mechanism is exogenous DNA internalized into target cells?
Endocytosis
Cell Culture - Lecture 8: Gene Transfer
Name three chemical procedures to introduce DNA into cells.
Cell Culture - Lecture 8: Gene Transfer
For what reason are transfection reagents positively charged?
To bind to negatively charged DNA backbone
Cell Culture - Lecture 8: Gene Transfer
What are three disadvantages about using Calcium Phosphate for transfection?
Cell Culture - Lecture 8: Gene Transfer
In the calcium phosphate transfection method, DNA is introduced into the cells as a ___ with calcium phosphate. The solid result can be observed under the microscope.
In the calcium phosphate transfection method, DNA is introduced into the cells as a co-precipitate with calcium phosphate. The solid result can be observed under the microscope.
Cell Culture - Lecture 8: Gene Transfer
Why is the DEAE Dextran transfection method only suitable for transient transfection?
DEAE Dextran has cytotoxic effects and must be reduced temporarily
Cell Culture - Lecture 8: Gene Transfer
Why does transfection using lipofection work better in rapidly growing and dividing cells?
There is no nuclear envelope to penetrate
Cell Culture - Lecture 8: Gene Transfer
How do you activate dendrimers?
Removal of tertiary amines
Cell Culture - Lecture 8: Gene Transfer
During microinjection, where do you inject the exogenous DNA?
Either into cytoplasm or directly into the nucleus
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