Cell Culture - Lecture 6: 3D Culture at Hochschule Bonn-Rhein-Sieg

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Study with flashcards and summaries for the course Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Define the term "confluency".

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are major advantages of 3D cultures compared to 2D monolayers?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are osteoblasts?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are osteoclasts?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which cells are couples by RANKL/RANK interactions?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Name three main characteristics of human tissue.

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

"2D monolayer cultures lack an extracellular matrix and intestinal fluid"

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Where in 2D cultures do you see differences in cells compared to in vivo?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which limitations in application do 2D cultures have, which 3D cultures do not have?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are the three main types of 3D cultures called?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

In an organ culture, what is the limiting factor for the explant size?

Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which gels are used for histotypic cultures?

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Exemplary flashcards for Cell Culture - Lecture 6: 3D Culture at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Cell Culture - Lecture 6: 3D Culture

Define the term "confluency".

Confluency is the percentage of the surface of a culture dish that is covered by adherent cells

→ For optimal cell growth, each cell line has an optimal confluency to prevent e.g. contact inhibition

Cell Culture - Lecture 6: 3D Culture

What are major advantages of 3D cultures compared to 2D monolayers?

  • allow cells to interact with surrounding 
  • creates a correct matrix for a specific microenvironment niche
  • complex transport mechanisms are imitated
  • in vivo drug efficacy and toxicity can be predicted more reliably

Cell Culture - Lecture 6: 3D Culture

What are osteoblasts?

bone forming cells

Cell Culture - Lecture 6: 3D Culture

What are osteoclasts?

bone reabsorbing cells

Cell Culture - Lecture 6: 3D Culture

Which cells are couples by RANKL/RANK interactions?

Osteoblast/stromal cells and osteoclasts

Cell Culture - Lecture 6: 3D Culture

Name three main characteristics of human tissue.

  • Consists of different cell types which interact with each other and exchange growth factors and other signalling molecules
  • Contains extracellular matrix providing mechanical stability and regulation of cell functions
  • Synthesizes intestinal fluid containing nutrients and biological effectors to maintain cell viability

Cell Culture - Lecture 6: 3D Culture

"2D monolayer cultures lack an extracellular matrix and intestinal fluid"

True

Cell Culture - Lecture 6: 3D Culture

Where in 2D cultures do you see differences in cells compared to in vivo?

  • Cellular morphology
  • Differentiation
  • Proliferation
  • Apoptosis
  • Gene Expression

Cell Culture - Lecture 6: 3D Culture

Which limitations in application do 2D cultures have, which 3D cultures do not have?

  • In vitro efficacy/toxicity testing for drug discovery
  • In vivo/ex vivo growth of tissue for regenerative medicine

Cell Culture - Lecture 6: 3D Culture

What are the three main types of 3D cultures called?

  • Organ culture
  • Histotypic culture
  • Organotypic culture

Cell Culture - Lecture 6: 3D Culture

In an organ culture, what is the limiting factor for the explant size?

Absence of vascular system

Cell Culture - Lecture 6: 3D Culture

Which gels are used for histotypic cultures?

  • Collagen gel
  • Matrigel

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