Biochemistry at Hochschule Bonn-Rhein-Sieg

Flashcards and summaries for Biochemistry at the Hochschule Bonn-Rhein-Sieg

Arrow Arrow

It’s completely free

studysmarter schule studium
d

4.5 /5

studysmarter schule studium
d

4.8 /5

studysmarter schule studium
d

4.5 /5

studysmarter schule studium
d

4.8 /5

Study with flashcards and summaries for the course Biochemistry at the Hochschule Bonn-Rhein-Sieg

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How does the Warburg-Christian method work?

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which amino acids do absorb the UV light at wavelength 280 nm?        

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What can be determined with the Warburg-Christian method? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How does Bradford method work? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are the advantages/disadvantages of Bradford method? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

What are enzymes? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How can enzyme activity be decreased?    

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How are enzyme inhibitors classified? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How do reversible inhibitors work? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

How do irreversible inhibitors work? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which two commonly used protein quantification assays do we use in the practical class? 

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Which enzyme is used in the practical and what is special about it? 

Your peers in the course Biochemistry at the Hochschule Bonn-Rhein-Sieg create and share summaries, flashcards, study plans and other learning materials with the intelligent StudySmarter learning app.

Get started now!

Flashcard Flashcard

Exemplary flashcards for Biochemistry at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Biochemistry

How does the Warburg-Christian method work?

- UV light is used to determine concentration of proteins by absorbtion of the light by amino acids at 280 nm

Biochemistry

Which amino acids do absorb the UV light at wavelength 280 nm?        
Tryptophan (Trp), Tyrosine (Tyr) and Phenylalanine (Phe)  

--> they are present in all proteins, so proteins have in general the property of light absorption 

Biochemistry

What can be determined with the Warburg-Christian method? 
- protein concentration
- contamination of a protein solution with nucleic acids 

Biochemistry

How does Bradford method work? 
- Uses the dye Coomassie Brilliant Blue G-250 which reacts with proteins by binding to basic and hydrophobic amino acids, which increases the absorption maximum from 465 nm to 595 nm
- A BSA standart curve is needed for comparison 

Biochemistry

What are the advantages/disadvantages of Bradford method? 
Advantage: Simple, fast, very sensitive

Disadvantage:
- The chromogenic rate varies among proteins. Contamination by a detergent may interfere with chromogenic reaction
- small linear range
- at low concentrations, SDS can mask interaction site on proteins
- at higher concentration, SDS can directly bind to coomassie, thereby generating a false-positive blue color

Biochemistry

What are enzymes? 
- catalysts which increase rate of reaction without being chemically changed themselves by stabilizing transition state (enzymes can enhance reaction by factors over 1000)

- highly specific for certain substrates and their activity depends on temperature, ionic strength, pH and buffer composition


Biochemistry

How can enzyme activity be decreased?    
By inhibitors

Biochemistry

How are enzyme inhibitors classified? 
They are classified based on their mode of action

Biochemistry

How do reversible inhibitors work? 
- They bind to the enzyme in a non-covalent fashion

Biochemistry

How do irreversible inhibitors work? 
By binding to enzyme in an covalent interaction. 

Biochemistry

Which two commonly used protein quantification assays do we use in the practical class? 
1. Warburg-Christian

2. Bradford

Biochemistry

Which enzyme is used in the practical and what is special about it? 
- Alkaline phosphatase
- It is Zn2+ dependent metalloenzyme

Sign up for free to see all flashcards and summaries for Biochemistry at the Hochschule Bonn-Rhein-Sieg

Singup Image Singup Image
Wave

Other courses from your degree program

For your degree program Biochemistry at the Hochschule Bonn-Rhein-Sieg there are already many courses on StudySmarter, waiting for you to join them. Get access to flashcards, summaries, and much more.

Back to Hochschule Bonn-Rhein-Sieg overview page

Molecular Genetics

Instrumental Analysis

IA 2 - HPLC

IA 3 - HPLC & RP-HPLC

Cell Culture

Immunology (Practical Course)

Immunology 🦠

Biochem 🧪

cell culture 🧫

Microbiology

Cell Culture - Lecture 1: Basics 1

Quality Assurance EXAM PREP

Cell Culture - Lecture 6: 3D Culture

Bioinformatics

Biotechnology

Biotechnology - Exam Preparation

ACR - Trial Design

quality assurance

Norwegisch

Physics

Organic Chemistry

Immunology - Kahoot (Q&A)

Immunology Exam Prep

Immunology: B-cell Activation and Secondary Lymphoid Organs

Immunology: Antibodies and their use in research

Immunology - Chapter 4: Phagocytes

Immunology - Chapter 11: B- and T-cell development

Immunology - Chapter 8: T-cell activation in secondary lymphoid tissues

Histology

medical microbiology

Swedish

Molecular Genetics

mol gen

Immunology

human biology/ histology

Immunology

Immunology

Organic Chemistry

Immunology

Cell culture - Cell lines

General Chemistry

Histology

What is StudySmarter?

What is StudySmarter?

StudySmarter is an intelligent learning tool for students. With StudySmarter you can easily and efficiently create flashcards, summaries, mind maps, study plans and more. Create your own flashcards e.g. for Biochemistry at the Hochschule Bonn-Rhein-Sieg or access thousands of learning materials created by your fellow students. Whether at your own university or at other universities. Hundreds of thousands of students use StudySmarter to efficiently prepare for their exams. Available on the Web, Android & iOS. It’s completely free.

Awards

Best EdTech Startup in Europe

Awards
Awards

EUROPEAN YOUTH AWARD IN SMART LEARNING

Awards
Awards

BEST EDTECH STARTUP IN GERMANY

Awards
Awards

Best EdTech Startup in Europe

Awards
Awards

EUROPEAN YOUTH AWARD IN SMART LEARNING

Awards
Awards

BEST EDTECH STARTUP IN GERMANY

Awards