Biochem 🧪 at Hochschule Bonn-Rhein-Sieg

Flashcards and summaries for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg

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Study with flashcards and summaries for the course Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

enzyme activity U

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

specific enzyme activity

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

alkaline phosphatase (AP)

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

metal binding sites of E. coli AP (M1, M2, M3)

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

effects of pH

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Ampholytes

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

ketose visualization after TLC

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Rf

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Raffinose

Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Dialysis

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Km

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V max

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Exemplary flashcards for Biochem 🧪 at the Hochschule Bonn-Rhein-Sieg on StudySmarter:

Biochem 🧪

enzyme activity U
μmol substrate conversion/ min amount of product formed within a certain time is proportional to the enzyme activity

Biochem 🧪

specific enzyme activity
U/mg protein

Biochem 🧪

alkaline phosphatase (AP)
homodimeric metalloenzyme (needs Zn2+ and Mg2+) stabilized by zinc ions (function can be abolished by the removal of zinc ions) magnesium does not activate AP but increases its activity (cofactor) catalyzes the hydrolysis of phosphate monoesters to form inorganic phosphate and alcohol must be kept on ice at all times! 140,000 Da

Biochem 🧪

metal binding sites of E. coli AP (M1, M2, M3)
M1: specific for Zn2+ (2 histidines) M2: slight preference for Zn2+ over Mg2+ (only 1 histidine) M3: less specific but Mg2+ is essential for the stability

Biochem 🧪

effects of pH
1. change of conformation of the enzyme by altering electrostatic interactions (breaking of non covalent interactions in the secondary/tertiary structure of proteins) --> binding of substrate to the enzyme (change of active site) --> catalytic activity of the enzyme (can still bind substrate, cannot perform reaction 2. ionization of substrates (substrate cannot bind properly anymore)

Biochem 🧪

Ampholytes
amphoteric molecules with acidic and basic groups, forming and buffering a pH gradient in an electric field used for isoelectric focussing

Biochem 🧪

ketose visualization after TLC
incubation with urea and hydrochloric acid at 100°C

Biochem 🧪

Rf
distance traveled by the spot Rf = ____________________________ distance traveled by the solvent

Biochem 🧪

Raffinose
a trisaccharide composed of D-galactose and sucrose) [α-D-glucose and β-D-fructose]

Biochem 🧪

Dialysis
separation according to molecular size diffusion through semipermeable membrane small molecules and ions may diffuse across the membrane e.g. for the purification/ desalting of proteins

Biochem 🧪

Km
substrate concentration at half maximum velocity

Biochem 🧪

V max
maximum velocity at saturating substrate concentration

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