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It is produced by T4 plasmid infected, and it is used to join together DNA molecules with complementary or bluny end in DNA cloning
. T4 DNA ligase
specialized plasmids, phages or hybrids which have been developed to make the construction of recombinant libraries and the identification of individual recombinant clones simpler
Vectors
accept inserts of about 10-15 kb long and is characterized by good ``packaging'' in the sense that it's very unlikely to lose its insert.
λ-phage
derivatives of bacteriophage lambda; linear DNA molecules, whose region can be replaced with foreign DNA without disrupting its life cycle
Phage
a group of replicas of all or part of a macromolecule (such as DNA or an Antibody
Cloning
used by LLNL in the context of shotgun sequencing. The size of inserts is about 1,500 bps, i.e. the fragments grown in M13 are ready to be sequenced
Bacteriophage M13
based on bacterial mini-F plasmids
Bacterial Artificial Chromosomes (BAC)
an extra chromosomal circular DNA molecule that combines features of plasmids and phage
Cosmids
Cloning limit: 75-300 kb.
Bacterial Artificial Chromosomes (BAC)
an artificial chromosome that contains telomeres, origin of replication, a yeast centromere, and a selectable marker for identification in yeast cells
Yeast Artificial Chromosomes (YAC)
an autonomous, an extra chromosomal circular DNA molecule that autonomously replicates inside the bacterial cell
Plasmid
DNA molecule that carries foreign DNA into a host cell, replicates inside a bacterial (or yeast) cell and produces many copies of itself and the foreign DNA
Cloning vector
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