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bilirubin (gmelin)
purple
tests for bile pigments based upon their oxidation by acids, with the formation of a series of colored derivatives like biliverdin (green) and bilirubin (purple).
Gmelin's test
W.hat is the principle of SAA?
precipitation of protein
with acidic acid and heat.
biliverdin
green
SAA means?
SALT ACETIC ACID TEST
Place in a boiling water bath
for?
5 minutes.
Process of SAA test in order:
centrifuge
transfer
add SAA or filtered urine
Boil
Cool and settle
W.at is the volume and duration of urine in centrifugation?
5 to 10 cc
5 minutes
How many SAA should be added to the urine supernatant?
1/3 of its volume of SAA solution
What urine can be used to SSA?
First morning urine
Random urine specimen
can also be used other than SAA soln.
Filtered urine
usually performed on dark yellow or orange urine. A urine containing bile will produce yellow foam chiefly from the pigment bilirubin.
Foam's test
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