Internship Interviews at Central Philippine University | Flashcards & Summaries

Lernmaterialien für Internship interviews an der Central Philippine University

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what are the two basic cell counting principles employed in most hematology analyzers?

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electrical impedance

optical scatter or detection.

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what are the advantages of automation?

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speed of performance, elimination of visual fatigue, improved precision.

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what is a cell histogram?

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1. A histogram utilizes impedance technology, and it is a representation of cell number versus one measured property, usually cell size. 2. Produced by plotting the number (of pulses) on the Y axis and the cell size on the X axis

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what are the types of error in automated methods?

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1. Instrumental Errors

2. Nature of the Specimen

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what are the basic components of hematology analyzers?

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1. Hydraulics

2. Pneumatics

3. Electrical Systems

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we must Repeat the analysis if:

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1. Rule of three failures on a normocytic sample (especially MCHC >37 g/dL) 

2. Any result outside linearity limits (++++) established by manufacturer – dilute into linearity range 

3. Unexplained delta check failures (e.g., results do not correlate with recent previous results, especially MCV)

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what are the Conditions that Cause Interference on Most Hematology Analyzers?

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Cold agglutinins

Lipemia, icterus

Hemolysis

Lysis-resistant RBCs*

Microcytes or schistocytes

Nucleated RBCs, megakaryocyte fragments, or micromegakaryoblasts

Platelet clumps

WBC . 100,000/mL

Leukemia, especially with chemotherapy

Old specimen

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give examples of *Lyse-resistant abnormal RBCs:

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sickle cells, target cells, hypochromic cells

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*MCH and MCHC spurious levels usually secondary to factors affecting:

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Hb and Hct

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give their parameters and correction:

Cold agglutinins

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TESTE DEIN WISSEN

↓ RBC, ↑ MCV, ↑ MCHC, grainy appearance

Warm specimen to 37° C and rerun

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TESTE DEIN WISSEN

errors in the nature of specimen

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TESTE DEIN WISSEN

a. Giant platelets may be counted as RBCs or WBCs 

b. Fragment of WBC cytoplasm may be counted as RBCs or platelets 

c. Agglutination of RBCs, WBCs or platelets will cause false-negative results for each count 

d. Platelet sattelitism falsely low platelet counts

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TESTE DEIN WISSEN

what are the rbc Shape Variations?

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Acanthocyte, Biscuit Cell, Bronze Elliptocyte

Codocyte, Dacryocyte, Drepanocyte

Echinocyte, Elliptocyte

Hb CC Crystals, Hb SC Crystals

Keratocyte*, Leptocyte, Ovalocytes

Schistocyte, Spherocyte, Stomatocyte

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Q:

what are the two basic cell counting principles employed in most hematology analyzers?

A:

electrical impedance

optical scatter or detection.

Q:

what are the advantages of automation?

A:

speed of performance, elimination of visual fatigue, improved precision.

Q:

what is a cell histogram?

A:

1. A histogram utilizes impedance technology, and it is a representation of cell number versus one measured property, usually cell size. 2. Produced by plotting the number (of pulses) on the Y axis and the cell size on the X axis

Q:

what are the types of error in automated methods?

A:

1. Instrumental Errors

2. Nature of the Specimen

Q:

what are the basic components of hematology analyzers?

A:

1. Hydraulics

2. Pneumatics

3. Electrical Systems

Mehr Karteikarten anzeigen
Q:

we must Repeat the analysis if:

A:

1. Rule of three failures on a normocytic sample (especially MCHC >37 g/dL) 

2. Any result outside linearity limits (++++) established by manufacturer – dilute into linearity range 

3. Unexplained delta check failures (e.g., results do not correlate with recent previous results, especially MCV)

Q:

what are the Conditions that Cause Interference on Most Hematology Analyzers?

A:

Cold agglutinins

Lipemia, icterus

Hemolysis

Lysis-resistant RBCs*

Microcytes or schistocytes

Nucleated RBCs, megakaryocyte fragments, or micromegakaryoblasts

Platelet clumps

WBC . 100,000/mL

Leukemia, especially with chemotherapy

Old specimen

Q:

give examples of *Lyse-resistant abnormal RBCs:

A:

sickle cells, target cells, hypochromic cells

Q:

*MCH and MCHC spurious levels usually secondary to factors affecting:

A:

Hb and Hct

Q:

give their parameters and correction:

Cold agglutinins

A:

↓ RBC, ↑ MCV, ↑ MCHC, grainy appearance

Warm specimen to 37° C and rerun

Q:

errors in the nature of specimen

A:

a. Giant platelets may be counted as RBCs or WBCs 

b. Fragment of WBC cytoplasm may be counted as RBCs or platelets 

c. Agglutination of RBCs, WBCs or platelets will cause false-negative results for each count 

d. Platelet sattelitism falsely low platelet counts

Q:

what are the rbc Shape Variations?

A:

Acanthocyte, Biscuit Cell, Bronze Elliptocyte

Codocyte, Dacryocyte, Drepanocyte

Echinocyte, Elliptocyte

Hb CC Crystals, Hb SC Crystals

Keratocyte*, Leptocyte, Ovalocytes

Schistocyte, Spherocyte, Stomatocyte

Internship interviews

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