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what is the basic method of gene cloning
how can a gene be used for reaesrch once it has been cloned
what is reverse genetics
manipulating gene to under or over produce to test biological function
how can genetic manipulation be used in medicine
how was gene analysis used to tackle Sars-Cov-2
what are restriction enzymes
recognise and cleave dna at certain cites
what is a palendromic sequence
where the top and bottom strand is the same 5'-->3'
used by restriction enzymes when cutting dna
what does it mean if a restriction enzyme leaves a blunt end
cuts straight down between 2 base pairs
how can dna ligase regoin cut dna
sticky ends- can form covalen bonds between 2 fragments with same end
blunt ends - can do it for these too but less efficient
how can a vector be used to clone dna
use modified plasids to replicate
like E. coli
what must plasmids that are being used gene cloning have
what is a clone of dna
duplicate that is genetically identical to the original section of dna
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