Recombinant DNA at Cardiff University | Flashcards & Summaries

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Lernmaterialien für Recombinant DNA an der Cardiff University

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what is the basic method of gene cloning 

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  • biological method of purification
  • dna inserted into vector then intorduced to host 
Lösung ausblenden
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how can a gene be used for reaesrch once it has been cloned 

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  • nucleotide seqeunce can be determined
  • AAs can be deduced
  • evo history of genes can be analysed
  • primers for PCR and be deduced 
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what is reverse genetics 

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manipulating gene to under or over produce to test biological function

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how can genetic manipulation be used in medicine

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  • can work out molecular basis of diseases
  • can seqeunce genomes of cells like cancer cells 
  • feotal dna analysed for prenantal testing
Lösung ausblenden
TESTE DEIN WISSEN

how was gene analysis used to tackle Sars-Cov-2

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  • determinde nucleotide seqeunce of genome
  • tested using pcr
  • tracks new variants with genome
Lösung ausblenden
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what are restriction enzymes 

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TESTE DEIN WISSEN

recognise and cleave dna at certain cites 

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what is a palendromic sequence 

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where the top and bottom strand is the same 5'-->3'

used by restriction enzymes when cutting dna

Lösung ausblenden
TESTE DEIN WISSEN

what does it mean if a restriction enzyme leaves a blunt end 

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cuts straight down between 2 base pairs 

Lösung ausblenden
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how can dna ligase regoin cut dna 

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sticky ends- can form covalen bonds between 2 fragments with same end 

blunt ends - can do it for these too but less efficient 

Lösung ausblenden
TESTE DEIN WISSEN

how can a vector be used to clone dna 

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TESTE DEIN WISSEN

use modified plasids to replicate 

like E. coli

Lösung ausblenden
TESTE DEIN WISSEN

what must plasmids that are being used gene cloning have

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TESTE DEIN WISSEN
  • unique restriction enzymes which can be ligated
  • Ori of replication
  • marker that idstinguishes plasmid and vector cells - usually antibiotic resistance gene
Lösung ausblenden
TESTE DEIN WISSEN

what is a clone of dna 

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duplicate that is genetically identical to the original section of dna

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  • 18884 Karteikarten
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Beispielhafte Karteikarten für deinen Recombinant DNA Kurs an der Cardiff University - von Kommilitonen auf StudySmarter erstellt!

Q:

what is the basic method of gene cloning 

A:
  • biological method of purification
  • dna inserted into vector then intorduced to host 
Q:

how can a gene be used for reaesrch once it has been cloned 

A:
  • nucleotide seqeunce can be determined
  • AAs can be deduced
  • evo history of genes can be analysed
  • primers for PCR and be deduced 
Q:

what is reverse genetics 

A:

manipulating gene to under or over produce to test biological function

Q:

how can genetic manipulation be used in medicine

A:
  • can work out molecular basis of diseases
  • can seqeunce genomes of cells like cancer cells 
  • feotal dna analysed for prenantal testing
Q:

how was gene analysis used to tackle Sars-Cov-2

A:
  • determinde nucleotide seqeunce of genome
  • tested using pcr
  • tracks new variants with genome
Mehr Karteikarten anzeigen
Q:

what are restriction enzymes 

A:

recognise and cleave dna at certain cites 

Q:

what is a palendromic sequence 

A:

where the top and bottom strand is the same 5'-->3'

used by restriction enzymes when cutting dna

Q:

what does it mean if a restriction enzyme leaves a blunt end 

A:

cuts straight down between 2 base pairs 

Q:

how can dna ligase regoin cut dna 

A:

sticky ends- can form covalen bonds between 2 fragments with same end 

blunt ends - can do it for these too but less efficient 

Q:

how can a vector be used to clone dna 

A:

use modified plasids to replicate 

like E. coli

Q:

what must plasmids that are being used gene cloning have

A:
  • unique restriction enzymes which can be ligated
  • Ori of replication
  • marker that idstinguishes plasmid and vector cells - usually antibiotic resistance gene
Q:

what is a clone of dna 

A:

duplicate that is genetically identical to the original section of dna

Recombinant DNA

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