Genetik at Bremen

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Denaturierung/ Initiation PCR

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Polytäne Chromosomen (Riesenchromosomen)

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Turner Syndrom

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Translokation

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Puff

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Mit welchem Verfahren wird die Absorption von Nukleinsäuren gemessen?

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Welche Optische Dichte hat DNA/ RNA/ Proteine?

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Giemsa Farbstoff

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Wanderung der DNA Gelelektrophorese

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Primer-Annealing PCR

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Ethidiumbromid bei der Gelelektrophorese

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Material Gelelektrophorese

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Exemplary flashcards for Genetik at the Bremen on StudySmarter:

Genetik

Denaturierung/ Initiation PCR

Trennung der doppelsträngigen DNA, Aufbrechen der WSBB der DNA bei ca. 94°C

Genetik

Polytäne Chromosomen (Riesenchromosomen)

• Entstehen durch Endomitose (Polytänisierung) → mehrere aufeinander folgende Replikationszyklen, ohne mitotische Teilung der Chromatiden
• Chromatiden bleiben aneinander in Bündeln hängen (DNA-Replikation ohne mitotische Teilung).
Ziel: Erhöhung der Syntheserate in der Zelle

Genetik

Turner Syndrom

45,X0

Genetik

Translokation

Chromosomensegment wird mit Segment eines anderen ausgetauscht

Genetik

Puff

Ort der erhöhten RNA-Synthese bei Riesen-
chromosomen von Dipteren

Genetik

Mit welchem Verfahren wird die Absorption von Nukleinsäuren gemessen?

Photometermessung
• DNA: 260 nm
• Protein: 280 nm

Genetik

Welche Optische Dichte hat DNA/ RNA/ Proteine?

• DNA → 1 OD = 50 ng/μl
• RNA → 1 OD = 40 ng/μl
• Oligos → 1 OD = 35 ng/μl

Genetik

Giemsa Farbstoff

färbt A/T-reiche DNA

Genetik

Wanderung der DNA Gelelektrophorese

Anion = neg.

Anode = pos
Kation = pos.
Kathode = neg

Die DNA wandert von Kathode (-) zu Anode (+), da die Seitengruppen (Phosphatgruppen) der DNA negativ geladen sind.
Ethidiumbromid wandert in die umgekehrte Richtung.

Genetik

Primer-Annealing PCR

Spezifische Anlagerung der Primer an die DNA (Template) bei ca. 60°C

Genetik

Ethidiumbromid bei der Gelelektrophorese

interkaliert in die DNA und fluoresziert bei UV-Bestrahlung 254 – 300 nm

Genetik

Material Gelelektrophorese

  • Ethidiumbromid/Gel Red
  • TAE-Puffer
  • Loading Buffer
  • DNA-Marker

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4. Genetik at

Medizinische Universität Wien

Genetik- Biologie at

Universität Würzburg

genetics at

Medical University Pleven

Genetica at

University of Aquila

Genetic at

University of Medicine and Pharmacy of Targu Mures

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