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Maxam–Gilbert sequencing
T + C
Hydrazine
Pyro sequencing is based on
as it relies on light detection based on a chain reaction when pyrophosphate is released when dNTPS incorporate to DNA.
“sequencing by synthesis principle
These enzymes recognize specific foreign DNA sequences and digest them to small fragments at specific sites named
(recognition sites)
chromogenic substrate
X-gal
The method is based on the cleavage of ssDNA at specific nucleotides using chemical reagents.
Maxam–Gilbert sequencing
The phosphate (5’ P) end of the DNA is removed and labeled by
the radiolabeled P³²
it is a real-time sequencing method based on the conversion of pyrophosphate groups, released during DNA elongation, into measurable light.
Pyrosequencing
A ddNTP is a human-made molecule that lacks a
hydroxyl group
Gene sequencing techniques?
Sanger sequencing 2) Maxam–Gilbert sequencing 3) Pyro-sequencing
Maxam–Gilbert sequencing
C
Hydrazine + 1.5M NaCl
Maxam–Gilbert sequencing
A + G
Formic acid
Maxam–Gilbert sequencing IS ALSO KNOWN AS
chemical cleavage method
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